Degradation of G alpha by the N-end rule pathway - PubMed (original) (raw)
. 1994 Sep 2;265(5177):1454-8.
doi: 10.1126/science.8073290.
Affiliations
- PMID: 8073290
- DOI: 10.1126/science.8073290
Degradation of G alpha by the N-end rule pathway
K Madura et al. Science. 1994.
Abstract
The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Overexpression of targeting components of the N-end rule pathway in Saccharomyces cerevisiae inhibited the growth of haploid but not diploid cells. This ploidy-dependent toxicity was shown to result from enhanced degradation of Gpa1, the alpha subunit (G alpha) of a heterotrimeric guanine nucleotide-binding protein (G protein) that regulates cell differentiation in response to mating pheromones. Sst2, a protein whose absence renders cells hypersensitive to pheromone, was essential for degradation of G alpha but not other N-end rule substrates, suggesting the involvement of an indirect, or trans-, targeting mechanism. G alpha degradation by the N-end rule pathway adds another regulatory dimension to the multitude of signaling functions mediated by G proteins.
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