hnRNA size and processing as related to different DNA content in two dipterans: Drosophila and Aedes - PubMed (original) (raw)
Comparative Study
hnRNA size and processing as related to different DNA content in two dipterans: Drosophila and Aedes
J Lengyel et al. Cell. 1975 Jul.
Abstract
The size of hnRNA transcripts and the fraction of hnRNA that is converted to mRNA in cell lines of Drosophila melanogaster and Aedes albopictus are compared. Both insects belong to the order Diptera, but Aedes has a 5-6 fold larger genome than does Drosophila. The Aedes line produces significantly (2-2.5 fold) larger hnRNA than does the Drosophila line, even though the two cell lines grow under similar conditions and produce mRNA of the same size and sequence complexity. These data suggest that within a given taxonomic order, the size of hnRNA increases with increasing genome size. The fraction of hnRNA converted to mRNA [cytoplasmic poly(A)+ RNA] has been measured for the two cell types by comparing initial rates of labeling of hnRNA with initial rates of appearance of labeled mRNA in the cytoplasm. While 20of the Drosophila hnRNA is converted to mRNA, only 3.3% of the Aedes hn %RNA is converted to mrRNA. The poly(A) content of the hnRNA from the two species is also different; Drosophila hnRNA has approximately three times as much poly(A) as does Aedes hnRNA. The data show-at least for these two species-that the average amount of hnRNA transcribed relative to the amount of mRNA formed increases as genome size increases. The data are consistent with the interpretation that more DNA is transcribed into hnRNA in Aedes, the organism with the larger genome, than in Drosophila.
Similar articles
- Relationship between nuclear and polysomal RNA populations of Achlya: a simple eucaryotic system.
Timberlake WE, Shumard DS, Goldberg RB. Timberlake WE, et al. Cell. 1977 Apr;10(4):623-32. doi: 10.1016/0092-8674(77)90095-2. Cell. 1977. PMID: 558829 - Concentrations of individual RNA sequences in polyadenylated nuclear and cytoplasmic RNA populations of Drosophila cells.
Biessmann H. Biessmann H. Nucleic Acids Res. 1980 Dec 20;8(24):6099-111. doi: 10.1093/nar/8.24.6099. Nucleic Acids Res. 1980. PMID: 6162158 Free PMC article. - The origin of mRNA and the structure of the mammalian chromosome.
Darnell JE Jr. Darnell JE Jr. Harvey Lect. 1973-1974;(69):1-47. Harvey Lect. 1973. PMID: 4376811 Review. No abstract available. - Messenger RNA metabolism of animal cells. Possible involvement of untranslated sequences and mRNA-associated proteins.
Greenberg JR. Greenberg JR. J Cell Biol. 1975 Feb;64(2):269-88. doi: 10.1083/jcb.64.2.269. J Cell Biol. 1975. PMID: 1090629 Free PMC article. Review.
Cited by
- Degradation of maternal poly(A)-containing RNA during early embryogenesis of an insect (Smittia spec., chironomidae, diptera).
Jäckle H. Jäckle H. Wilehm Roux Arch Dev Biol. 1979 Jun;187(2):179-193. doi: 10.1007/BF00848270. Wilehm Roux Arch Dev Biol. 1979. PMID: 28304929 - Black beetle virus: messenger for protein B is a subgenomic viral RNA.
Friesen PD, Rueckert RR. Friesen PD, et al. J Virol. 1982 Jun;42(3):986-95. doi: 10.1128/JVI.42.3.986-995.1982. J Virol. 1982. PMID: 16789225 Free PMC article. - Heat-shock proteins are associated with hnRNA in Drosophila melanogaster tissue culture cells.
Kloetzel PM, Bautz EK. Kloetzel PM, et al. EMBO J. 1983;2(5):705-10. doi: 10.1002/j.1460-2075.1983.tb01488.x. EMBO J. 1983. PMID: 16453451 Free PMC article. - Further evidence that the majority of primary nuclear RNA transcripts in mammalian cells do not contribute to mRNA.
Salditt-Georgieff M, Darnell JE Jr. Salditt-Georgieff M, et al. Mol Cell Biol. 1982 Jun;2(6):701-7. doi: 10.1128/mcb.2.6.701-707.1982. Mol Cell Biol. 1982. PMID: 14582165 Free PMC article. - Structural organization of nascent transcripts and hnRNA molecules in amphibian oocytes.
Sommerville J, Scheer U. Sommerville J, et al. Mol Biol Rep. 1981 May 22;7(1-3):53-6. doi: 10.1007/BF00778733. Mol Biol Rep. 1981. PMID: 7254207
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases