ERG gene is translocated in an Ewing's sarcoma cell line - PubMed (original) (raw)
ERG gene is translocated in an Ewing's sarcoma cell line
T Dunn et al. Cancer Genet Cytogenet. 1994 Aug.
Abstract
Ewing's sarcoma (ES) and related neoplasias are characterized by the reciprocal translocation, t(11;22)(q24;q12). The translocation has been reported to generate a fusion gene between the EWS (a previously undescribed gene on chromosome 22) and FLI1 genes. We report a similar translocation of EWS and FLI1 in an Askin's tumor cell line (SK-NM-C). Further, we describe an alternative translocation in an ES cell line (#5838) in which the 5' end of the EWS gene is juxtaposed to the 3' end of the ERG gene. The ERG gene is on chromosome 21, but no microscopically visible changes in chromosome 21 were observed. Elevated steady state levels of the EWS/ERG fusion gene transcript were detected in the #5838 cell line. This is the first report of a structural alteration of ERG in human cancer. Also, it confirms a general mechanism of generating putative oncogenic fusion genes by placing an ETS DNA binding domain in direct proximity to the carboxy terminus domain (CTD) related region of the EWS gene.
Similar articles
- EWS-erg and EWS-Fli1 fusion transcripts in Ewing's sarcoma and primitive neuroectodermal tumors with variant translocations.
Giovannini M, Biegel JA, Serra M, Wang JY, Wei YH, Nycum L, Emanuel BS, Evans GA. Giovannini M, et al. J Clin Invest. 1994 Aug;94(2):489-96. doi: 10.1172/JCI117360. J Clin Invest. 1994. PMID: 8040301 Free PMC article. - Cloning of the entire FLI1 gene, disrupted by the Ewing's sarcoma translocation breakpoint on 11q24, in a yeast artificial chromosome.
Selleri L, Giovannini M, Romo A, Zucman J, Delattre O, Thomas G, Evans GA. Selleri L, et al. Cytogenet Cell Genet. 1994;67(2):129-36. doi: 10.1159/000133813. Cytogenet Cell Genet. 1994. PMID: 8039423 - Identification of various exon combinations of the ews/fli1 translocation: an optimized RT-PCR method for paraffin embedded tissue -- a report by the CWS-study group.
Stegmaier S, Leuschner I, Aakcha-Rudel E, Münch P, Kazanowska B, Bekassy A, Treuner J, Koscielniak E. Stegmaier S, et al. Klin Padiatr. 2004 Nov-Dec;216(6):315-22. doi: 10.1055/s-2004-832338. Klin Padiatr. 2004. PMID: 15565546 - Ewing's sarcoma oncoprotein EWS-FLI1: the perfect target without a therapeutic agent.
Uren A, Toretsky JA. Uren A, et al. Future Oncol. 2005 Aug;1(4):521-8. doi: 10.2217/14796694.1.4.521. Future Oncol. 2005. PMID: 16556028 Review. - Complex rearrangement of chromosomes 19, 21, and 22 in Ewing sarcoma involving a novel reciprocal inversion-insertion mechanism of EWS-ERG fusion gene formation: a case analysis and literature review.
Maire G, Brown CW, Bayani J, Pereira C, Gravel DH, Bell JC, Zielenska M, Squire JA. Maire G, et al. Cancer Genet Cytogenet. 2008 Mar;181(2):81-92. doi: 10.1016/j.cancergencyto.2007.11.002. Cancer Genet Cytogenet. 2008. PMID: 18295659 Review.
Cited by
- Immune-related gene IL17RA as a diagnostic marker in osteoporosis.
Deng YJ, Li Z, Wang B, Li J, Ma J, Xue X, Tian X, Liu QC, Zhang Y, Yuan B. Deng YJ, et al. Front Genet. 2023 Aug 4;14:1219894. doi: 10.3389/fgene.2023.1219894. eCollection 2023. Front Genet. 2023. PMID: 37600656 Free PMC article. - Evaluating the RIST Molecular-Targeted Regimen in a Three-Dimensional Neuroblastoma Spheroid Cell Culture Model.
Kaess C, Matthes M, Gross J, Waetzig R, Heise T, Corbacioglu S, Sommer G. Kaess C, et al. Cancers (Basel). 2023 Mar 14;15(6):1749. doi: 10.3390/cancers15061749. Cancers (Basel). 2023. PMID: 36980635 Free PMC article. - miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.
Wei Y, Peng J, He S, Huang H, Lin L, Zhu Q, Ye L, Li T, Zhang X, Gao Y, Zheng X. Wei Y, et al. J Cancer. 2020 May 18;11(15):4453-4463. doi: 10.7150/jca.44441. eCollection 2020. J Cancer. 2020. PMID: 32489464 Free PMC article. - Use of Fluorescence In Situ Hybridization (FISH) in Diagnosis and Tailored Therapies in Solid Tumors.
Chrzanowska NM, Kowalewski J, Lewandowska MA. Chrzanowska NM, et al. Molecules. 2020 Apr 17;25(8):1864. doi: 10.3390/molecules25081864. Molecules. 2020. PMID: 32316657 Free PMC article. Review. - The oncogene ERG: a key factor in prostate cancer.
Adamo P, Ladomery MR. Adamo P, et al. Oncogene. 2016 Jan 28;35(4):403-14. doi: 10.1038/onc.2015.109. Epub 2015 Apr 27. Oncogene. 2016. PMID: 25915839 Review.
MeSH terms
LinkOut - more resources
Full Text Sources
Molecular Biology Databases