Accumulation of unintegrated circular viral DNA in monocytes and growth-arrested T cells following infection with HIV-1 - PubMed (original) (raw)

Review

Accumulation of unintegrated circular viral DNA in monocytes and growth-arrested T cells following infection with HIV-1

S Sonza et al. J Leukoc Biol. 1994 Sep.

Abstract

Cytocidal retrovirus infection is characterized by rapid accumulation of unintegrated viral DNA forms. These are thought to be generated by multiple rounds of reinfection and have been suggested to play a central role in cytopathogenesis. Here we have reviewed the work done in this area with HIV-1, mostly using acutely and chronically infected T cell and monocytic cell lines and in some cases T cells blocked at S phase of the cell cycle by aphidicolin treatment. To these studies, we have compared our findings with HIV-1 infected primary peripheral blood monocyte-derived macrophages and untreated and growth-arrested MT-2 cells, two biologically disparate cell populations. Using 1- and 2-long terminal repeat (LTR) circular forms as indicators of unintegrated viral DNA, we found similar rapid accumulation in both untreated and growth-arrested MT-2 cells. In contrast, we found much lower levels in monocyte/macrophages. Our findings suggest that accumulation of unintegrated viral DNA does not require virus production and reinfection in growth-arrested T cells. The significantly lower levels found in monocyte/macrophages may reflect superinfection resistance, allowing the maintenance of a persistent infection.

PubMed Disclaimer

Cited by

Human immunodeficiency virus type 1 RNA Levels in different regions of human brain: quantification using real-time reverse transcriptase-polymerase chain reaction.
Kumar AM, Borodowsky I, Fernandez B, Gonzalez L, Kumar M. Kumar AM, et al. J Neurovirol. 2007 Jun;13(3):210-24. doi: 10.1080/13550280701327038. J Neurovirol. 2007. PMID: 17613711
Distribution and fate of HIV-1 unintegrated DNA species: a comprehensive update.
Hamid FB, Kim J, Shin CG. Hamid FB, et al. AIDS Res Ther. 2017 Feb 16;14(1):9. doi: 10.1186/s12981-016-0127-6. AIDS Res Ther. 2017. PMID: 28209198 Free PMC article. Review.
Human immunodeficiency virus type 1 replication is blocked prior to reverse transcription and integration in freshly isolated peripheral blood monocytes.
Sonza S, Maerz A, Deacon N, Meanger J, Mills J, Crowe S. Sonza S, et al. J Virol. 1996 Jun;70(6):3863-9. doi: 10.1128/JVI.70.6.3863-3869.1996. J Virol. 1996. PMID: 8648722 Free PMC article.
Circular forms of unintegrated human immunodeficiency virus type 1 DNA and high levels of viral protein expression: association with dementia and multinucleated giant cells in the brains of patients with AIDS.
Teo I, Veryard C, Barnes H, An SF, Jones M, Lantos PL, Luthert P, Shaunak S. Teo I, et al. J Virol. 1997 Apr;71(4):2928-33. doi: 10.1128/JVI.71.4.2928-2933.1997. J Virol. 1997. PMID: 9060651 Free PMC article.
Host hindrance to HIV-1 replication in monocytes and macrophages.
Bergamaschi A, Pancino G. Bergamaschi A, et al. Retrovirology. 2010 Apr 7;7:31. doi: 10.1186/1742-4690-7-31. Retrovirology. 2010. PMID: 20374633 Free PMC article. Review.

Accumulation of unintegrated circular viral DNA in monocytes and growth-arrested T cells following infection with HIV-1 - PubMed (original) (raw)