Interleukin 13 induces interleukin 4-independent IgG4 and IgE synthesis and CD23 expression by human B cells - PubMed (original) (raw)

Interleukin 13 induces interleukin 4-independent IgG4 and IgE synthesis and CD23 expression by human B cells

J Punnonen et al. Proc Natl Acad Sci U S A. 1993.

Abstract

Recently the cDNA encoding interleukin 13 (IL-13), a T-cell-derived cytokine, was cloned and expressed. The present study demonstrates that IL-13 induces IgG4 and IgE synthesis by human B cells. IL-13-induced IgG4 and IgE synthesis by unfractionated peripheral blood mononuclear cells and highly purified B cells cultured in the presence of activated CD4+ T cells or their membranes. IL-13-induced IgG4 and IgE synthesis is IL-4-independent, since it was not affected by neutralizing anti-IL-4 monoclonal antibody. Highly purified, surface IgD+ B cells could also be induced to produce IgG4 and IgE by IL-13, indicating that the production of these isotypes reflected IgG4 and IgE switching and not a selective outgrowth of committed B cells. IL-4 and IL-13 added together at optimal concentrations had no additive or synergistic effect, suggesting that common signaling pathways may be involved. This notion is supported by the observation that IL-13, like IL-4, induced CD23 expression on B cells and enhanced CD72, surface IgM, and class II major histocompatibility complex antigen expression. In addition, like IL-4, IL-13 induced germ-line IgE heavy-chain gene transcription in highly purified B cells. Collectively, our data indicate that IL-13 is another T-cell-derived cytokine that, in addition to IL-4, efficiently directs naive human B cells to switch to IgG4 and IgE production.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Nucleic Acids Res. 1986 Jul 11;14(13):5275-94 - PubMed
    1. Immunology. 1984 Oct;53(2):197-205 - PubMed
    1. Science. 1987 May 22;236(4804):944-7 - PubMed
    1. J Exp Med. 1987 Jun 1;165(6):1459-67 - PubMed
    1. J Immunol. 1987 Aug 15;139(4):1142-7 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources