Synaptic transmission persists in synaptotagmin mutants of Drosophila - PubMed (original) (raw)
Comparative Study
. 1993 Jul 2;73(7):1281-90.
doi: 10.1016/0092-8674(93)90356-u.
Affiliations
- PMID: 8100740
- DOI: 10.1016/0092-8674(93)90356-u
Comparative Study
Synaptic transmission persists in synaptotagmin mutants of Drosophila
A DiAntonio et al. Cell. 1993.
Abstract
Synaptotagmin is one of the major integral membrane proteins of synaptic vesicles. It has been postulated to dock vesicles to their release sites, to act as the Ca2+ sensor for the release process, and to be a fusion protein during exocytosis. To clarify the function of this protein, we have undertaken a genetic analysis of the synaptotagmin gene in Drosophila. We have identified five lethal alleles of synaptotagmin, at least one of which lacks detectable protein. Surprisingly, however, many embryos homozygous for this null allele hatch and, as larvae, crawl, feed, and respond to stimuli. Electrophysiological recordings in embryonic cultures confirmed that synaptic transmission persists in the null allele. Therefore, synaptotagmin is not absolutely required for the regulated exocytosis of synaptic vesicles. The lethality of synaptotagmin in late first instar larvae is probably due to a perturbation of transmission that leaves the main apparatus for vesicle docking and fusion intact.
Similar articles
- Absence of synaptotagmin disrupts excitation-secretion coupling during synaptic transmission.
Broadie K, Bellen HJ, DiAntonio A, Littleton JT, Schwarz TL. Broadie K, et al. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10727-31. doi: 10.1073/pnas.91.22.10727. Proc Natl Acad Sci U S A. 1994. PMID: 7938019 Free PMC article. - Calcium dependence of neurotransmitter release and rate of spontaneous vesicle fusions are altered in Drosophila synaptotagmin mutants.
Littleton JT, Stern M, Perin M, Bellen HJ. Littleton JT, et al. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10888-92. doi: 10.1073/pnas.91.23.10888. Proc Natl Acad Sci U S A. 1994. PMID: 7971978 Free PMC article. - Drosophila synaptotagmin I null mutants survive to early adulthood.
Loewen CA, Mackler JM, Reist NE. Loewen CA, et al. Genesis. 2001 Sep;31(1):30-6. doi: 10.1002/gene.10002. Genesis. 2001. PMID: 11668675 - p65-Synaptotagmin: a docking-fusion protein in synaptic vesicle exocytosis?
Popoli M. Popoli M. Neuroscience. 1993 May;54(2):323-8. doi: 10.1016/0306-4522(93)90255-e. Neuroscience. 1993. PMID: 8336827 Review. No abstract available. - Is synaptotagmin the calcium sensor?
Yoshihara M, Adolfsen B, Littleton JT. Yoshihara M, et al. Curr Opin Neurobiol. 2003 Jun;13(3):315-23. doi: 10.1016/s0959-4388(03)00063-1. Curr Opin Neurobiol. 2003. PMID: 12850216 Review.
Cited by
- Open Frontiers in Neural Cell Type Investigations; Lessons From Caenorhabditis elegans and Beyond, Toward a Multimodal Integration.
Rapti G. Rapti G. Front Neurosci. 2022 Mar 7;15:787753. doi: 10.3389/fnins.2021.787753. eCollection 2021. Front Neurosci. 2022. PMID: 35321480 Free PMC article. Review. - A Sexually Dimorphic Olfactory Neuron Mediates Fixed Action Transition during Courtship Ritual in Drosophila melanogaster.
Tanaka NK, Hirao T, Chida H, Ejima A. Tanaka NK, et al. J Neurosci. 2021 Nov 24;41(47):9732-9741. doi: 10.1523/JNEUROSCI.1168-21.2021. Epub 2021 Oct 14. J Neurosci. 2021. PMID: 34649953 Free PMC article. - Function of Drosophila Synaptotagmins in membrane trafficking at synapses.
Quiñones-Frías MC, Littleton JT. Quiñones-Frías MC, et al. Cell Mol Life Sci. 2021 May;78(9):4335-4364. doi: 10.1007/s00018-021-03788-9. Epub 2021 Feb 22. Cell Mol Life Sci. 2021. PMID: 33619613 Free PMC article. Review. - The role of the C2A domain of synaptotagmin 1 in asynchronous neurotransmitter release.
Shields MC, Bowers MR, Kramer HL, Fulcer MM, Perinet LC, Metz MJ, Reist NE. Shields MC, et al. PLoS One. 2020 May 14;15(5):e0232991. doi: 10.1371/journal.pone.0232991. eCollection 2020. PLoS One. 2020. PMID: 32407359 Free PMC article. - The C2A domain of synaptotagmin is an essential component of the calcium sensor for synaptic transmission.
Bowers MR, Reist NE. Bowers MR, et al. PLoS One. 2020 Feb 7;15(2):e0228348. doi: 10.1371/journal.pone.0228348. eCollection 2020. PLoS One. 2020. PMID: 32032373 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous