Epithelial cell proliferation and islet neogenesis in IFN-g transgenic mice - PubMed (original) (raw)

Epithelial cell proliferation and islet neogenesis in IFN-g transgenic mice

D Gu et al. Development. 1993 May.

Abstract

We have identified a model system for the study of pancreatic islet development and regeneration in transgenic mice bearing the interferon-gamma (IFN-g) gene expressed in the pancreatic islets. Previous studies showed that the locally produced IFN-g causes lymphocyte infiltration and islet cell destruction. Here we demonstrate that new islet cells are formed continuously from duct cells as evidenced by (1) the dramatic proliferation of duct cells, (2) the appearance of primitive cells and (3) their subsequent differentiation to endocrine cells. The IFN-g induced islet neogenesis is similar to embryonic islet morphogenesis and offers a model system for studying factors modulating islet development. Additionally, the duct cells occasionally transdifferentiate to gastrointestinal-like cell types and hepatocytes. These results underscore the lymphokine's ability to initiate a complex 'transdifferentiation' pathway, providing a window for understanding lineage interrelationships within a terminally differentiated structure.

PubMed Disclaimer

Cited by

Transgenic Expression of a Single Transcription Factor Pdx1 Induces Transdifferentiation of Pancreatic Acinar Cells to Endocrine Cells in Adult Mice.
Miyazaki S, Tashiro F, Miyazaki J. Miyazaki S, et al. PLoS One. 2016 Aug 15;11(8):e0161190. doi: 10.1371/journal.pone.0161190. eCollection 2016. PLoS One. 2016. PMID: 27526291 Free PMC article.
In vivo reprogramming of Sox9+ cells in the liver to insulin-secreting ducts.
Banga A, Akinci E, Greder LV, Dutton JR, Slack JM. Banga A, et al. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15336-41. doi: 10.1073/pnas.1201701109. Epub 2012 Sep 4. Proc Natl Acad Sci U S A. 2012. PMID: 22949652 Free PMC article.
Pancreatic β-Cell Mass as a Pharmacologic Target in Diabetes.
Hanley S. Hanley S. Mcgill J Med. 2009 Nov 16;12(2):51. Mcgill J Med. 2009. PMID: 21264047 Free PMC article.
Gene expression profiling of a mouse model of pancreatic islet dysmorphogenesis.
Wilding Crawford L, Tweedie Ables E, Oh YA, Boone B, Levy S, Gannon M. Wilding Crawford L, et al. PLoS One. 2008 Feb 20;3(2):e1611. doi: 10.1371/journal.pone.0001611. PLoS One. 2008. PMID: 18297134 Free PMC article.
Pancreatic expression of keratinocyte growth factor leads to differentiation of islet hepatocytes and proliferation of duct cells.
Krakowski ML, Kritzik MR, Jones EM, Krahl T, Lee J, Arnush M, Gu D, Sarvetnick N. Krakowski ML, et al. Am J Pathol. 1999 Mar;154(3):683-91. doi: 10.1016/S0002-9440(10)65315-1. Am J Pathol. 1999. PMID: 10079246 Free PMC article.

Epithelial cell proliferation and islet neogenesis in IFN-g transgenic mice - PubMed (original) (raw)