Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain - PubMed (original) (raw)
Comparative Study
Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain
N Patel et al. Proc Natl Acad Sci U S A. 1993.
Abstract
An additional genetic locus in Caenorhabditis elegans, unc-116, was identified in a screen for mutations resulting in defective locomotion. unc-116 was cloned by use of a transposon insertion mutant and the physical and genetic map of the genome. The cDNA sequence predicts an 815-amino acid protein. Based upon sequence comparison and secondary structure predictions, unc-116 encodes all three domains of the kinesin heavy chain: the motor, stalk, and tail. While the motor and tail domains have a high degree of identity to the equivalent domains of cloned kinesin heavy chains, the rodII domain of the stalk is significantly shorter than those previously reported and is not predicted to form a coiled-coil alpha-helix. Analysis of mutational defects in two C. elegans genes encoding anterograde motor molecules, unc-116 and unc-104, should provide insight into the in vivo functions of these members of the kinesin heavy chain superfamily.
Similar articles
- Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to the external environment.
Tabish M, Siddiqui ZK, Nishikawa K, Siddiqui SS. Tabish M, et al. J Mol Biol. 1995 Mar 31;247(3):377-89. doi: 10.1006/jmbi.1994.0146. J Mol Biol. 1995. PMID: 7714894 - Kinesin light chain isoforms in Caenorhabditis elegans.
Fan J, Amos LA. Fan J, et al. J Mol Biol. 1994 Jul 29;240(5):507-12. doi: 10.1006/jmbi.1994.1465. J Mol Biol. 1994. PMID: 8046755 - The C. elegans unc-104 gene encodes a putative kinesin heavy chain-like protein.
Otsuka AJ, Jeyaprakash A, García-Añoveros J, Tang LZ, Fisk G, Hartshorne T, Franco R, Born T. Otsuka AJ, et al. Neuron. 1991 Jan;6(1):113-22. doi: 10.1016/0896-6273(91)90126-k. Neuron. 1991. PMID: 1846075 - Metazoan motor models: kinesin superfamily in C. elegans.
Siddiqui SS. Siddiqui SS. Traffic. 2002 Jan;3(1):20-8. doi: 10.1034/j.1600-0854.2002.30104.x. Traffic. 2002. PMID: 11872139 Review. - Expression cloning with pan kinesin antibodies.
Ginkel LM, Wordeman L. Ginkel LM, et al. Methods Mol Biol. 2001;164:21-41. doi: 10.1385/1-59259-069-1:21. Methods Mol Biol. 2001. PMID: 11217609 Review. No abstract available.
Cited by
- The role of kinesin-1 in neuronal dense core vesicle transport, locomotion and lifespan regulation in C. elegans.
Gavrilova A, Boström A, Korabel N, Fedotov S, Poulin GB, Allan VJ. Gavrilova A, et al. J Cell Sci. 2024 Sep 1;137(17):jcs262148. doi: 10.1242/jcs.262148. Epub 2024 Sep 6. J Cell Sci. 2024. PMID: 39171448 Free PMC article. - UNC-116 and UNC-16 function with the NEKL-3 kinase to promote axon targeting.
Drozd CJ, Quinn CC. Drozd CJ, et al. Development. 2023 Sep 15;150(18):dev201654. doi: 10.1242/dev.201654. Epub 2023 Sep 27. Development. 2023. PMID: 37756604 Free PMC article. - JIP3 interacts with dynein and kinesin-1 to regulate bidirectional organelle transport.
Celestino R, Gama JB, Castro-Rodrigues AF, Barbosa DJ, Rocha H, d'Amico EA, Musacchio A, Carvalho AX, Morais-Cabral JH, Gassmann R. Celestino R, et al. J Cell Biol. 2022 Aug 1;221(8):e202110057. doi: 10.1083/jcb.202110057. Epub 2022 Jul 13. J Cell Biol. 2022. PMID: 35829703 Free PMC article. - UNC-16 alters DLK-1 localization and negatively regulates actin and microtubule dynamics in Caenorhabditis elegans regenerating neurons.
Kulkarni SS, Sabharwal V, Sheoran S, Basu A, Matsumoto K, Hisamoto N, Ghosh-Roy A, Koushika SP. Kulkarni SS, et al. Genetics. 2021 Nov 5;219(3):iyab139. doi: 10.1093/genetics/iyab139. Genetics. 2021. PMID: 34740241 Free PMC article. - GRDN-1/Girdin regulates dendrite morphogenesis and cilium position in two specialized sensory neuron types in C. elegans.
Nechipurenko I, Lavrentyeva S, Sengupta P. Nechipurenko I, et al. Dev Biol. 2021 Apr;472:38-51. doi: 10.1016/j.ydbio.2020.12.022. Epub 2021 Jan 16. Dev Biol. 2021. PMID: 33460640 Free PMC article.
References
- Nucleic Acids Res. 1987 Feb 25;15(4):1353-61 - PubMed
- Proc Natl Acad Sci U S A. 1986 Feb;83(4):907-11 - PubMed
- J Biol Chem. 1992 Oct 5;267(28):20457-64 - PubMed
- Science. 1990 Jul 6;249(4964):42-7 - PubMed
- Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources