Subcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors in a speckled pattern in the nucleoplasm - PubMed (original) (raw)

Subcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors in a speckled pattern in the nucleoplasm

K H Kalland et al. J Virol. 1994 Mar.

Abstract

The human immunodeficiency virus type 1 (HIV-1) Rev (regulator of virion protein expression) protein exemplifies a new type of posttranscriptional regulation. One main function of Rev is to increase the cytoplasmic expression of unspliced and incompletely spliced retroviral mRNAs from which viral structural proteins are made. In that way, Rev is essential in order to complete the retroviral life cycle. The biology of Rev in the host cell has remained elusive. In this study, a complex distribution of Rev in single cells was found. Rev was found in the cytoplasm, in a perinuclear zone, in the nucleoplasm, and in the nucleoli. In the nucleoplasm, Rev colocalized in a speckled pattern with host cell factors known to assemble on nascent transcripts. Those factors are involved in the processing of heterogeneous RNA to spliced mRNA in the nucleoplasm of all cells. The distribution of Rev was dependent only on Rev and host cell interactions, since neither the Rev target RNA nor other HIV proteins were expressed in the cells. Rev was found in the same subcellular compartments of cells treated for extended periods with cycloheximide, an inhibitor of protein synthesis. This finding implies that Rev shuttles continuously between cytoplasmic and nucleoplasmic compartments. The results suggest a potential role for Rev both in the RNA-splicing process and in the nucleocytoplasmic transport of Rev-dependent HIV mRNA.

PubMed Disclaimer

Cited by

Structure, Activity and Function of the Protein Arginine Methyltransferase 6.
Gupta S, Kadumuri RV, Singh AK, Chavali S, Dhayalan A. Gupta S, et al. Life (Basel). 2021 Sep 11;11(9):951. doi: 10.3390/life11090951. Life (Basel). 2021. PMID: 34575100 Free PMC article. Review.
Proteolytic activity assayed by subcellular localization switching of a substrate.
Szilvay AM, Sarria SV, Mannelqvist M, Aasland R, Furnes C. Szilvay AM, et al. Biochem Biophys Rep. 2016 Jul 16;8:23-28. doi: 10.1016/j.bbrep.2016.07.011. eCollection 2016 Dec. Biochem Biophys Rep. 2016. PMID: 28955937 Free PMC article.
Dynamics of the human and viral m(6)A RNA methylomes during HIV-1 infection of T cells.
Lichinchi G, Gao S, Saletore Y, Gonzalez GM, Bansal V, Wang Y, Mason CE, Rana TM. Lichinchi G, et al. Nat Microbiol. 2016 Feb 22;1:16011. doi: 10.1038/nmicrobiol.2016.11. Nat Microbiol. 2016. PMID: 27572442 Free PMC article.
Dynamic Post-Transcriptional Regulation of HIV-1 Gene Expression.
Kula A, Marcello A. Kula A, et al. Biology (Basel). 2012 Jul 3;1(2):116-33. doi: 10.3390/biology1020116. Biology (Basel). 2012. PMID: 24832221 Free PMC article.
Nuclear trafficking of retroviral RNAs and Gag proteins during late steps of replication.
Stake MS, Bann DV, Kaddis RJ, Parent LJ. Stake MS, et al. Viruses. 2013 Nov 18;5(11):2767-95. doi: 10.3390/v5112767. Viruses. 2013. PMID: 24253283 Free PMC article. Review.

References

1. J Cell Biol. 1987 May;104(5):1143-56 - PubMed
1. Eur J Biochem. 1987 Oct 15;168(2):461-7 - PubMed
1. J Virol. 1988 Feb;62(2):655-8 - PubMed
1. Science. 1988 Feb 19;239(4842):910-3 - PubMed
1. Cell. 1988 Mar 11;52(5):655-64 - PubMed

Subcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors in a speckled pattern in the nucleoplasm - PubMed (original) (raw)