Dynamic actin structures stabilized by profilin - PubMed (original) (raw)

Dynamic actin structures stabilized by profilin

T Finkel et al. Proc Natl Acad Sci U S A. 1994.

Abstract

We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.

PubMed Disclaimer

Similar articles

• Effects of profilin and profilactin on actin structure and function in living cells.
  Cao LG, Babcock GG, Rubenstein PA, Wang YL. Cao LG, et al. J Cell Biol. 1992 Jun;117(5):1023-9. doi: 10.1083/jcb.117.5.1023. J Cell Biol. 1992. PMID: 1577865 Free PMC article.
• Interaction of plant profilin with mammalian actin.
  Giehl K, Valenta R, Rothkegel M, Ronsiek M, Mannherz HG, Jockusch BM. Giehl K, et al. Eur J Biochem. 1994 Dec 1;226(2):681-9. doi: 10.1111/j.1432-1033.1994.tb20096.x. Eur J Biochem. 1994. PMID: 8001585
• Plant and animal profilins are functionally equivalent and stabilize microfilaments in living animal cells.
  Rothkegel M, Mayboroda O, Rohde M, Wucherpfennig C, Valenta R, Jockusch BM. Rothkegel M, et al. J Cell Sci. 1996 Jan;109 ( Pt 1):83-90. doi: 10.1242/jcs.109.1.83. J Cell Sci. 1996. PMID: 8834793
• Signal transduction and the actin cytoskeleton: the roles of MARCKS and profilin.
  Aderem A. Aderem A. Trends Biochem Sci. 1992 Oct;17(10):438-43. doi: 10.1016/0968-0004(92)90016-3. Trends Biochem Sci. 1992. PMID: 1455513 Review.
• Profilins as regulators of actin dynamics.
  Schlüter K, Jockusch BM, Rothkegel M. Schlüter K, et al. Biochim Biophys Acta. 1997 Nov 27;1359(2):97-109. doi: 10.1016/s0167-4889(97)00100-6. Biochim Biophys Acta. 1997. PMID: 9409807 Review. No abstract available.

Cited by

• Platelet PlA2 polymorphism and thromboembolic events: from inherited risk to pharmacogenetics.
  Goldschmidt-Clermont PJ, Roos CM, Cooke GE. Goldschmidt-Clermont PJ, et al. J Thromb Thrombolysis. 1999 Aug;8(2):89-103. doi: 10.1023/a:1008954916972. J Thromb Thrombolysis. 1999. PMID: 10436139 Review. No abstract available.
• Profilin-1 is a negative regulator of mammary carcinoma aggressiveness.
  Zou L, Jaramillo M, Whaley D, Wells A, Panchapakesa V, Das T, Roy P. Zou L, et al. Br J Cancer. 2007 Nov 19;97(10):1361-71. doi: 10.1038/sj.bjc.6604038. Epub 2007 Oct 16. Br J Cancer. 2007. PMID: 17940506 Free PMC article.
• The Actin Cytoskeleton in Myelinating Cells.
  Brown TL, Macklin WB. Brown TL, et al. Neurochem Res. 2020 Mar;45(3):684-693. doi: 10.1007/s11064-019-02753-0. Epub 2019 Mar 7. Neurochem Res. 2020. PMID: 30847860 Free PMC article. Review.
• A balance of capping protein and profilin functions is required to regulate actin polymerization in Drosophila bristle.
  Hopmann R, Miller KG. Hopmann R, et al. Mol Biol Cell. 2003 Jan;14(1):118-28. doi: 10.1091/mbc.e02-05-0300. Mol Biol Cell. 2003. PMID: 12529431 Free PMC article.
• Profilin1 facilitates staurosporine-triggered apoptosis by stabilizing the integrin β1-actin complex in breast cancer cells.
  Yao W, Yu X, Fang Z, Yin P, Zhao C, Li N, Wang L, Li Z, Zha X. Yao W, et al. J Cell Mol Med. 2012 Apr;16(4):824-35. doi: 10.1111/j.1582-4934.2011.01369.x. J Cell Mol Med. 2012. PMID: 21692986 Free PMC article.

References

1. 1. J Cell Sci. 1992 Apr;101 ( Pt 4):731-43 - PubMed
2. 1. Science. 1987 Oct 30;238(4827):638-44 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4831-5 - PubMed
4. 1. J Biol Chem. 1988 Apr 25;263(12):5910-5 - PubMed
5. 1. Cell Regul. 1990 Nov;1(12):937-50 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Miscellaneous

  • NCI CPTAC Assay Portal