Human immunodeficiency virus type 1 Nef associates with a cellular serine kinase in T lymphocytes - PubMed (original) (raw)
Human immunodeficiency virus type 1 Nef associates with a cellular serine kinase in T lymphocytes
E T Sawai et al. Proc Natl Acad Sci U S A. 1994.
Abstract
With T-cell lines constitutively expressing Nef from the SF2 strain of human immunodeficiency virus type 1 (HIV-1SF2) in the form of a hybrid CD8-Nef fusion protein or T-cell lines chronically infected with HIV-1SF2, a cellular serine kinase was found that specifically associates with Nef. Proteins of 62 kDa and 72 kDa, which coimmunoprecipitated with Nef, were phosphorylated in in vitro kinase assays. This Nef-associated serine kinase activity was not blocked by inhibitors of protein kinase C or protein kinase A and was lost when Nef was truncated at amino acid 94 or 99. These findings present evidence that a serine kinase activity is associated with Nef expressed in human T lymphocytes.
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References
- Virology. 1992 May;188(1):391-5 - PubMed
- Virology. 1992 Mar;187(1):46-55 - PubMed
- AIDS Res Hum Retroviruses. 1991 Dec;7(12):1015-20 - PubMed
- Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6634-8 - PubMed
- AIDS Res Hum Retroviruses. 1992 May;8(5):537-43 - PubMed
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