Sequence determination and mutational analysis of the lly locus of Legionella pneumophila - PubMed (original) (raw)

Sequence determination and mutational analysis of the lly locus of Legionella pneumophila

E Wintermeyer et al. Infect Immun. 1994 Mar.

Abstract

The lly (legiolysin) locus codes for a 39-kDa protein which confers hemolysis, pigment production, and fluorescence on recombinant Escherichia coli K-12 clones carrying the lly gene. The nucleotide sequences of the lly genes from two Legionella pneumophila isolates were determined. The lly loci exhibited identical nucleotide sequences. They contained open reading frames of 348 amino acid residues, encoding proteins with a deduced molecular mass of 38.9 kDa. N-terminal amino acid sequencing further confirmed that the Lly protein corresponds to the open reading frame sequenced. The amino acid sequence of the Lly protein exhibits a high degree of homology with the sequences of the MelA protein responsible for melanin production in the freshwater bacterium Shewanella colwelliana and the 4-hydroxyphenylpyruvate dioxygenase of Pseudomonas spp. 4-Hydroxyphenylpyruvate dioxygenase is involved in the degradation of aromatic amino acids in various organisms. An Lly-negative mutant of L. pneumophila Philadelphia I derivative JR32 and an Lly-positive transcomplementant were constructed. The Lly-negative mutant lost the ability to produce brown pigment and to confer fluorescence but retained hemolysis. Introduction of a plasmid carrying the lly locus restored pigment production and fluorescence. Intracellular survival of L. pneumophila in U937 macrophage-like cells and in Acanthamoeba castellanii was not affected by mutagenization of the lly locus.

PubMed Disclaimer

Cited by

Components of the Legionella pneumophila flagellar regulon contribute to multiple virulence traits, including lysosome avoidance and macrophage death.
Molofsky AB, Shetron-Rama LM, Swanson MS. Molofsky AB, et al. Infect Immun. 2005 Sep;73(9):5720-34. doi: 10.1128/IAI.73.9.5720-5734.2005. Infect Immun. 2005. PMID: 16113289 Free PMC article.
Isolation of antibiotics turbomycin a and B from a metagenomic library of soil microbial DNA.
Gillespie DE, Brady SF, Bettermann AD, Cianciotto NP, Liles MR, Rondon MR, Clardy J, Goodman RM, Handelsman J. Gillespie DE, et al. Appl Environ Microbiol. 2002 Sep;68(9):4301-6. doi: 10.1128/AEM.68.9.4301-4306.2002. Appl Environ Microbiol. 2002. PMID: 12200279 Free PMC article.
De novo synthesis of Legionella pneumophila antigens during intracellular growth in phagocytic cells.
Susa M, Hacker J, Marre R. Susa M, et al. Infect Immun. 1996 May;64(5):1679-84. doi: 10.1128/iai.64.5.1679-1684.1996. Infect Immun. 1996. PMID: 8613378 Free PMC article.
Cloning and sequence analysis of a novel hemolysin gene (vllY) from Vibrio vulnificus.
Chang TM, Chuang YC, Su JH, Chang MC. Chang TM, et al. Appl Environ Microbiol. 1997 Oct;63(10):3851-7. doi: 10.1128/aem.63.10.3851-3857.1997. Appl Environ Microbiol. 1997. PMID: 9327548 Free PMC article.
Minimization of the Legionella pneumophila genome reveals chromosomal regions involved in host range expansion.
O'Connor TJ, Adepoju Y, Boyd D, Isberg RR. O'Connor TJ, et al. Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):14733-40. doi: 10.1073/pnas.1111678108. Epub 2011 Aug 22. Proc Natl Acad Sci U S A. 2011. PMID: 21873199 Free PMC article.

References

1. Annu Rev Genet. 1992;26:51-69 - PubMed
1. FEMS Microbiol Lett. 1990 Nov;60(3):253-7 - PubMed
1. J Clin Microbiol. 1990 Sep;28(9):2145-7 - PubMed
1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
1. Gene. 1983 Oct;24(2-3):299-308 - PubMed

Sequence determination and mutational analysis of the lly locus of Legionella pneumophila - PubMed (original) (raw)