Vesicular stomatitis virus glycoprotein is sorted and concentrated during export from the endoplasmic reticulum - PubMed (original) (raw)
Vesicular stomatitis virus glycoprotein is sorted and concentrated during export from the endoplasmic reticulum
W E Balch et al. Cell. 1994.
Abstract
Newly synthesized proteins are believed to move from the endoplasmic reticulum (ER) to the Golgi by bulk flow, and sorting is assumed to occur exclusively in the trans-Golgi network (TGN). Using quantitative immunoelectron microscopy, we demonstrate that vesicular stomatitis virus glycoprotein (VSV-G) is sorted from resident ER proteins and concentrated 5- to 10-fold in 40-80 nm vesicles during vesicle budding from the ER. Accumulation of VSV-G in pre-Golgi vesicular carriers is the only detectable concentration step in its transport to the TGN. From these results, it is apparent that export from the ER is not exclusively mediated by bulk flow. The ER exerts an unanticipated level of control to insure selective and efficient entry of mature protein into the secretory pathway.
Similar articles
- Morphological analysis of the transfer of VSV ts-045 G glycoprotein from the endoplasmic reticulum to the intermediate compartment in vero cells.
Lotti LV, Torrisi MR, Erra MC, Bonatti S. Lotti LV, et al. Exp Cell Res. 1996 Sep 15;227(2):323-31. doi: 10.1006/excr.1996.0281. Exp Cell Res. 1996. PMID: 8831570 - Export of protein from the endoplasmic reticulum is regulated by a diacylglycerol/phorbol ester binding protein.
Fabbri M, Bannykh S, Balch WE. Fabbri M, et al. J Biol Chem. 1994 Oct 28;269(43):26848-57. J Biol Chem. 1994. PMID: 7523413 - Morphological and functional association of Sec22b/ERS-24 with the pre-Golgi intermediate compartment.
Zhang T, Wong SH, Tang BL, Xu Y, Hong W. Zhang T, et al. Mol Biol Cell. 1999 Feb;10(2):435-53. doi: 10.1091/mbc.10.2.435. Mol Biol Cell. 1999. PMID: 9950687 Free PMC article. - Analysis of protein transport through the Golgi in a reconstituted cell-free system.
Wattenberg BW. Wattenberg BW. J Electron Microsc Tech. 1991 Feb;17(2):150-64. doi: 10.1002/jemt.1060170204. J Electron Microsc Tech. 1991. PMID: 1901603 Review. - Roles for microtubules and kinesin in membrane traffic between the endoplasmic reticulum and the Golgi complex.
Lippincott-Schwartz J, Cole NB. Lippincott-Schwartz J, et al. Biochem Soc Trans. 1995 Aug;23(3):544-8. doi: 10.1042/bst0230544. Biochem Soc Trans. 1995. PMID: 8566412 Review. No abstract available.
Cited by
- Passive diffusion accounts for the majority of intracellular nanovesicle transport.
Sittewelle M, Royle SJ. Sittewelle M, et al. Life Sci Alliance. 2023 Oct 19;7(1):e202302406. doi: 10.26508/lsa.202302406. Print 2024 Jan. Life Sci Alliance. 2023. PMID: 37857498 Free PMC article. - Sequence-directed concentration of G protein-coupled receptors in COPII vesicles.
Xu X, Lambert NA, Wu G. Xu X, et al. iScience. 2023 Sep 19;26(10):107969. doi: 10.1016/j.isci.2023.107969. eCollection 2023 Oct 20. iScience. 2023. PMID: 37810244 Free PMC article. - TMED10 mediates the loading of neosynthesised Sonic Hedgehog in COPII vesicles for efficient secretion and signalling.
Bare Y, Matusek T, Vriz S, Deffieu MS, Thérond PP, Gaudin R. Bare Y, et al. Cell Mol Life Sci. 2023 Aug 25;80(9):266. doi: 10.1007/s00018-023-04918-1. Cell Mol Life Sci. 2023. PMID: 37624561 Free PMC article. - Cargo selection in endoplasmic reticulum-to-Golgi transport and relevant diseases.
Tang VT, Ginsburg D. Tang VT, et al. J Clin Invest. 2023 Jan 3;133(1):e163838. doi: 10.1172/JCI163838. J Clin Invest. 2023. PMID: 36594468 Free PMC article. Review. - Nanopore Assay Reveals Cell-Type-Dependent Gene Expression of Vesicular Stomatitis Indiana Virus and Differential Host Cell Response.
Kakuk B, Kiss AA, Torma G, Csabai Z, Prazsák I, Mizik M, Megyeri K, Tombácz D, Boldogkői Z. Kakuk B, et al. Pathogens. 2021 Sep 15;10(9):1196. doi: 10.3390/pathogens10091196. Pathogens. 2021. PMID: 34578228 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous