Human immunodeficiency virus (HIV) nef-specific cytotoxic T lymphocytes in noninfected heterosexual contact of HIV-infected patients - PubMed (original) (raw)
Human immunodeficiency virus (HIV) nef-specific cytotoxic T lymphocytes in noninfected heterosexual contact of HIV-infected patients
P Langlade-Demoyen et al. J Clin Invest. 1994 Mar.
Abstract
We report on the detection of HIV-specific cytotoxic T lymphocytes (CTL) among 23 regular partners of HIV-infected individuals. 15 of the 46 individuals enrolled in the study were positive for HLA-A2.1 typing. Among the 23 contacts studied, 7 were seropositive and 16 were seronegative on repeated tests. None of the 16 seronegative contacts were positive for p24 antigenemia nor were they positive by the lymphocytes coculture assay, although, in two instances HIV-1 DNA could be detected by PCR (in one case using a gag SK 38/39 primer, and in the other using a primer for the pol P3/P4 primer). These two individuals remained seronegative for 18 and 36 mo, respectively. HIV-specific cytotoxicity was performed in the 15 HLA-A2.1 subjects (7 indexes, 2 seropositive contacts, and 6 seronegative contacts) and in 4 HLA-matched HIV negative donors. CTL specific for env, gag, or nef proteins could not be detected in unstimulated bulk cultures of peripheral blood lymphocytes in any of the six seronegative contacts. However, using a limiting dilution assay we found an usually high frequency of HIV nef-specific CTL precursors (CTLp) for HIV env and gag was very similar to that observed in seronegative HLA-matched healthy donors. Because no presence of HIV could be demonstrated in these individuals, these findings argue against the possibility of a silent HIV infection and suggest that a CTL response against nef may be involved in a rapid and effective clearance of the virus after sexual exposure.
Comment in
- Immune responses to human immunodeficiency virus type 1 in exposed but uninfected individuals: protection or chance?
Looney DJ. Looney DJ. J Clin Invest. 1994 Mar;93(3):920. doi: 10.1172/JCI117096. J Clin Invest. 1994. PMID: 8132777 Free PMC article. No abstract available.
Similar articles
- Multiple subsets of HIV-specific cytotoxic T lymphocytes in humans and in mice.
Chenciner N, Michel F, Dadaglio G, Langlade-Demoyen P, Hoffenbach A, Leroux A, Garcia-Pons F, Rautmann G, Guy B, Guillon JM, et al. Chenciner N, et al. Eur J Immunol. 1989 Sep;19(9):1537-44. doi: 10.1002/eji.1830190904. Eur J Immunol. 1989. PMID: 2676560 - Cytolytic T lymphocytes (CTLs) from HIV-1 subtype C-infected Indian patients recognize CTL epitopes from a conserved immunodominant region of HIV-1 Gag and Nef.
Thakar MR, Bhonge LS, Lakhashe SK, Shankarkumar U, Sane SS, Kulkarni SS, Mahajan BA, Paranjape RS. Thakar MR, et al. J Infect Dis. 2005 Sep 1;192(5):749-59. doi: 10.1086/432547. Epub 2005 Jul 27. J Infect Dis. 2005. PMID: 16088824 - Carboxyl-terminal and central regions of human immunodeficiency virus-1 NEF recognized by cytotoxic T lymphocytes from lymphoid organs. An in vitro limiting dilution analysis.
Hadida F, Parrot A, Kieny MP, Sadat-Sowti B, Mayaud C, Debre P, Autran B. Hadida F, et al. J Clin Invest. 1992 Jan;89(1):53-60. doi: 10.1172/JCI115585. J Clin Invest. 1992. PMID: 1370302 Free PMC article. - Do cytotoxic T lymphocytes clear some HIV/SIV infections?
Ada G. Ada G. J Med Primatol. 1996 Jun;25(3):158-62. doi: 10.1111/j.1600-0684.1996.tb00012.x. J Med Primatol. 1996. PMID: 8892036 Review. - Cytotoxic T lymphocytes in human immunodeficiency virus infection: regulator genes.
Riviere Y, Robertson MN, Buseyne F. Riviere Y, et al. Curr Top Microbiol Immunol. 1994;189:65-74. doi: 10.1007/978-3-642-78530-6_4. Curr Top Microbiol Immunol. 1994. PMID: 7924437 Review. No abstract available.
Cited by
- Determinant of HIV-1 mutational escape from cytotoxic T lymphocytes.
Yang OO, Sarkis PT, Ali A, Harlow JD, Brander C, Kalams SA, Walker BD. Yang OO, et al. J Exp Med. 2003 May 19;197(10):1365-75. doi: 10.1084/jem.20022138. Epub 2003 May 12. J Exp Med. 2003. PMID: 12743169 Free PMC article. - Higher macrophage inflammatory protein (MIP)-1alpha and MIP-1beta levels from CD8+ T cells are associated with asymptomatic HIV-1 infection.
Cocchi F, DeVico AL, Yarchoan R, Redfield R, Cleghorn F, Blattner WA, Garzino-Demo A, Colombini-Hatch S, Margolis D, Gallo RC. Cocchi F, et al. Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13812-7. doi: 10.1073/pnas.240469997. Proc Natl Acad Sci U S A. 2000. PMID: 11095721 Free PMC article. - Risk and management of blood-borne infections in health care workers.
Beltrami EM, Williams IT, Shapiro CN, Chamberland ME. Beltrami EM, et al. Clin Microbiol Rev. 2000 Jul;13(3):385-407. doi: 10.1128/CMR.13.3.385. Clin Microbiol Rev. 2000. PMID: 10885983 Free PMC article. Review. - A longitudinal study of feline immunodeficiency virus-specific cytotoxic T lymphocytes in experimentally infected cats, using antigen-specific induction.
Beatty JA, Willett BJ, Gault EA, Jarrett O. Beatty JA, et al. J Virol. 1996 Sep;70(9):6199-206. doi: 10.1128/JVI.70.9.6199-6206.1996. J Virol. 1996. PMID: 8709246 Free PMC article.
References
- J Immunol. 1988 Sep 15;141(6):1949-57 - PubMed
- Lancet. 1988 Sep 3;2(8610):538-41 - PubMed
- Transfusion. 1989 Mar-Apr;29(3):265-7 - PubMed
- N Engl J Med. 1989 Jun 1;320(22):1458-62 - PubMed
- Lancet. 1989 Sep 16;2(8664):637-40 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials