PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates - PubMed (original) (raw)

PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates

W M Barnes. Proc Natl Acad Sci U S A. 1994.

Abstract

A target length limitation to PCR amplification of DNA has been identified and addressed. Concomitantly, the base-pair fidelity, the ability to use PCR products as primers, and the maximum yield of target fragment were increased. These improvements were achieved by the combination of a high level of an exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase, Klentaq1, with a very low level of a thermostable DNA polymerase exhibiting a 3'-exonuclease activity (Pfu, Vent, or Deep Vent). At least 35 kb can be amplified to high yields from 1 ng of lambda DNA template.

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References

1. 1. Anal Biochem. 1992 Apr;202(1):46-9 - PubMed
2. 1. Mol Microbiol. 1992 May;6(9):1211-7 - PubMed
3. 1. Nature. 1993 Apr 22;362(6422):709-15 - PubMed
4. 1. PCR Methods Appl. 1993 May;2(4):275-87 - PubMed
5. 1. J Bacteriol. 1971 Oct;108(1):5-9 - PubMed

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