Purification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L - PubMed (original) (raw)
Comparative Study
. 1994 Jan-Feb;49(1-2):26-32.
doi: 10.1515/znc-1994-1-205.
Affiliations
- PMID: 8148006
- DOI: 10.1515/znc-1994-1-205
Free article
Comparative Study
Purification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L
A Baumert et al. Z Naturforsch C J Biosci. 1994 Jan-Feb.
Free article
Abstract
Acridone synthase has been purified from cell suspension cultures of Ruta graveolens using a combination of gel filtration and ion exchange chromatography. The purified enzyme has an apparent molecular weight of 69 kDa on gel filtration and a subunit structure on SDS-PAGE of 40 kDa. The apparent Km-values are 10.64 microM and 32.8 microM for N-methylanthraniloyl-CoA and malonyl-CoA, respectively. Tryptic digestion of the homogeneous acridone synthase was performed. Seven of the peptides were chosen for microsequencing. The homology of the amino acid sequences from this particular polypeptide and corresponding peptides from chalcone synthase 3 from garden pea amounted to 76%.
Similar articles
- Native acridone synthases I and II from Ruta graveolens L. form homodimers.
Lukacin R, Springob K, Urbanke C, Ernwein C, Schröder G, Schröder J, Matern U. Lukacin R, et al. FEBS Lett. 1999 Apr 1;448(1):135-40. doi: 10.1016/s0014-5793(99)00355-5. FEBS Lett. 1999. PMID: 10217426 - Molecular cloning and heterologous expression of acridone synthase from elicited Ruta graveolens L. cell suspension cultures.
Junghanns KT, Kneusel RE, Baumert A, Maier W, Gröger D, Matern U. Junghanns KT, et al. Plant Mol Biol. 1995 Feb;27(4):681-92. doi: 10.1007/BF00020222. Plant Mol Biol. 1995. PMID: 7727746 - Specificities of functionally expressed chalcone and acridone synthases from Ruta graveolens.
Springob K, Lukacin R, Ernwein C, Gröning I, Matern U. Springob K, et al. Eur J Biochem. 2000 Nov;267(22):6552-9. doi: 10.1046/j.1432-1327.2000.01746.x. Eur J Biochem. 2000. PMID: 11054106 - Purification and characterization of sinapine synthase from seeds of Brassica napus.
Vogt T, Aebershold R, Ellis B. Vogt T, et al. Arch Biochem Biophys. 1993 Feb 1;300(2):622-8. doi: 10.1006/abbi.1993.1086. Arch Biochem Biophys. 1993. PMID: 8382030 - Differential regulation and distribution of acridone synthase in Ruta graveolens.
Junghanns KT, Kneusel RE, Gröger D, Matern U. Junghanns KT, et al. Phytochemistry. 1998 Sep;49(2):403-11. doi: 10.1016/s0031-9422(98)00104-6. Phytochemistry. 1998. PMID: 9747538
Cited by
- An Overview of the Medicinally Important Plant Type III PKS Derived Polyketides.
Bisht R, Bhattacharyya A, Shrivastava A, Saxena P. Bisht R, et al. Front Plant Sci. 2021 Oct 14;12:746908. doi: 10.3389/fpls.2021.746908. eCollection 2021. Front Plant Sci. 2021. PMID: 34721474 Free PMC article. Review. - Duplication and functional divergence in the chalcone synthase gene family of Asteraceae: evolution with substrate change and catalytic simplification.
Helariutta Y, Kotilainen M, Elomaa P, Kalkkinen N, Bremer K, Teeri TH, Albert VA. Helariutta Y, et al. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9033-8. doi: 10.1073/pnas.93.17.9033. Proc Natl Acad Sci U S A. 1996. PMID: 8799149 Free PMC article. - Chalcone synthase-like genes active during corolla development are differentially expressed and encode enzymes with different catalytic properties in Gerbera hybrida (Asteraceae).
Helariutta Y, Elomaa P, Kotilainen M, Griesbach RJ, Schröder J, Teeri TH. Helariutta Y, et al. Plant Mol Biol. 1995 Apr;28(1):47-60. doi: 10.1007/BF00042037. Plant Mol Biol. 1995. PMID: 7787187 - Exploiting the Biosynthetic Potential of Type III Polyketide Synthases.
Lim YP, Go MK, Yew WS. Lim YP, et al. Molecules. 2016 Jun 22;21(6):806. doi: 10.3390/molecules21060806. Molecules. 2016. PMID: 27338328 Free PMC article. Review. - Evolutionary and functional analysis of mulberry type III polyketide synthases.
Li H, Liang J, Chen H, Ding G, Ma B, He N. Li H, et al. BMC Genomics. 2016 Aug 4;17:540. doi: 10.1186/s12864-016-2843-7. BMC Genomics. 2016. PMID: 27487946 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases