Improved techniques for the preparation of bacterial lipopolysaccharides - PubMed (original) (raw)
Comparative Study
Improved techniques for the preparation of bacterial lipopolysaccharides
K G Johnson et al. Can J Microbiol. 1976 Jan.
Abstract
Liopolysaccharides were prepared from six organisms by the use of two cell-disruption procedures before conventional phenol-water extraction. Disruption of cells by grinding with glass beads or by digestion with hen egg white lysozyme before phenol extraction facilitated rapid purification and greater yields of lipopolysaccharide. Pretreatment of cells with lysozyme in the presence of ethylenediaminetetraacetic acid was the most efficient method in terms of lipopolysaccharide yield and ease of preparation. Increase in lipopolysaccharide yield achieved by use of the lysozyme method, compared with the conventional phenol extraction, varied from 1.7- to 12.4-fold. Preparations were designated as pure according to several criteria and were judged not to have undergone changes as a result of prephenol extraction procedures.
Similar articles
- Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures.
Morrison DC, Leive L. Morrison DC, et al. J Biol Chem. 1975 Apr 25;250(8):2911-19. J Biol Chem. 1975. PMID: 804483 - Degradative effect of phenol on endotoxin and lipopolysaccharide preparations from Serratia marcescens.
Tsang JC, Wang CS, Alaupovic P. Tsang JC, et al. J Bacteriol. 1974 Feb;117(2):786-95. doi: 10.1128/jb.117.2.786-795.1974. J Bacteriol. 1974. PMID: 4204442 Free PMC article. - A method for purification of bacterial R-type lipopolysaccharides (lipooligosaccharides).
Wu LH, Tsai CM, Frasch CE. Wu LH, et al. Anal Biochem. 1987 Feb 1;160(2):281-9. doi: 10.1016/0003-2697(87)90048-0. Anal Biochem. 1987. PMID: 2883909 - The effect of ethylenediaminetetraacetate and lysozyme on isolated lipopolysaccharide from Pseudomonas aeruginosa.
Michaels GB, Eagon RG. Michaels GB, et al. Proc Soc Exp Biol Med. 1966 Jul;122(3):866-8. doi: 10.3181/00379727-122-31273. Proc Soc Exp Biol Med. 1966. PMID: 4958464 No abstract available. - Interaction of bacteria and fungi with lectins and lectin-like substances.
Pistole TG. Pistole TG. Annu Rev Microbiol. 1981;35:85-112. doi: 10.1146/annurev.mi.35.100181.000505. Annu Rev Microbiol. 1981. PMID: 7027908 Review. No abstract available.
Cited by
- The Lipid A from the Lipopolysaccharide of the Phototrophic Bacterium Rhodomicrobium vannielii ATCC 17100 Revisited.
Komaniecka I, Susniak K, Choma A, Heine H, Holst O. Komaniecka I, et al. Int J Mol Sci. 2020 Dec 29;22(1):258. doi: 10.3390/ijms22010258. Int J Mol Sci. 2020. PMID: 33383833 Free PMC article. - Lipid A from Oligotropha carboxidovorans Lipopolysaccharide That Contains Two Galacturonic Acid Residues in the Backbone and Malic Acid A Tertiary Acyl Substituent.
Choma A, Zamłyńska K, Mazur A, Pastuszka A, Kaczyński Z, Komaniecka I. Choma A, et al. Int J Mol Sci. 2020 Oct 27;21(21):7991. doi: 10.3390/ijms21217991. Int J Mol Sci. 2020. PMID: 33121154 Free PMC article. - Effect of the Extracellular Vesicle RNA Cargo From Uropathogenic Escherichia coli on Bladder Cells.
Dauros-Singorenko P, Hong J, Swift S, Phillips A, Blenkiron C. Dauros-Singorenko P, et al. Front Mol Biosci. 2020 Sep 25;7:580913. doi: 10.3389/fmolb.2020.580913. eCollection 2020. Front Mol Biosci. 2020. PMID: 33102527 Free PMC article. - Conjugation of Meningococcal Lipooligosaccharides Through Their Non-Reducing Terminus Results in Improved Induction a Protective Immune Response.
Mieszała M, Jennings HJ, Drab M, Gamian A. Mieszała M, et al. Arch Immunol Ther Exp (Warsz). 2019 Aug;67(4):237-248. doi: 10.1007/s00005-019-00542-9. Epub 2019 Apr 27. Arch Immunol Ther Exp (Warsz). 2019. PMID: 31030218 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources