Adhesion forces between individual ligand-receptor pairs - PubMed (original) (raw)

. 1994 Apr 15;264(5157):415-7.

doi: 10.1126/science.8153628.

Affiliations

• PMID: 8153628
• DOI: 10.1126/science.8153628

Adhesion forces between individual ligand-receptor pairs

E L Florin et al. Science. 1994.

Abstract

The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 +/- 20 piconewtons for biotin and 85 +/- 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.

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