Evidence that random and imprinted Xist expression is controlled by preemptive methylation - PubMed (original) (raw)
Evidence that random and imprinted Xist expression is controlled by preemptive methylation
D P Norris et al. Cell. 1994.
Abstract
The mouse Xist gene is expressed exclusively from the inactive X chromosome and may control the initiation of X inactivation. We show that in somatic tissues the 5' end of the silent Xist allele on the active X chromosome is fully methylated, while the expressed allele on the inactive X is completely unmethylated. In tissues that undergo imprinted paternal Xist expression and imprinted X inactivation, the paternal Xist allele is unmethylated, and the silent maternal allele is fully methylated. In the male germline, a developmentally regulated demethylation of Xist occurs at the onset of meiosis and is retained in mature spermatozoa. This may be the cause of imprinted expression of the paternal Xist allele. A role for methylation in the control of Xist expression is further supported by the finding that in differentiating embryonic stem cells during the initiation of X inactivation, differential methylation of Xist alleles precedes the onset of Xist expression.
Similar articles
- Methylation of the mouse Xist gene in sperm and eggs correlates with imprinted Xist expression and paternal X-inactivation.
Zuccotti M, Monk M. Zuccotti M, et al. Nat Genet. 1995 Mar;9(3):316-20. doi: 10.1038/ng0395-316. Nat Genet. 1995. PMID: 7773296 - Mosaic methylation of Xist gene before chromosome inactivation in undifferentiated female mouse embryonic stem and embryonic germ cells.
Sado T, Tada T, Takagi N. Sado T, et al. Dev Dyn. 1996 Apr;205(4):421-34. doi: 10.1002/(SICI)1097-0177(199604)205:4<421::AID-AJA6>3.0.CO;2-K. Dev Dyn. 1996. PMID: 8901053 - X inactivation: Tsix and Xist as yin and yang.
Mlynarczyk SK, Panning B. Mlynarczyk SK, et al. Curr Biol. 2000 Dec 14-28;10(24):R899-903. doi: 10.1016/s0960-9822(00)00847-2. Curr Biol. 2000. PMID: 11137025 Review. - Xist and X chromosome inactivation.
Kay GF. Kay GF. Mol Cell Endocrinol. 1998 May 25;140(1-2):71-6. doi: 10.1016/s0303-7207(98)00032-x. Mol Cell Endocrinol. 1998. PMID: 9722171 Review.
Cited by
- Effects of DNMT1 and HDAC inhibitors on gene-specific methylation reprogramming during porcine somatic cell nuclear transfer.
Xu W, Li Z, Yu B, He X, Shi J, Zhou R, Liu D, Wu Z. Xu W, et al. PLoS One. 2013 May 31;8(5):e64705. doi: 10.1371/journal.pone.0064705. Print 2013. PLoS One. 2013. PMID: 23741375 Free PMC article. - X-chromosome inactivation in monkey embryos and pluripotent stem cells.
Tachibana M, Ma H, Sparman ML, Lee HS, Ramsey CM, Woodward JS, Sritanaudomchai H, Masterson KR, Wolff EE, Jia Y, Mitalipov SM. Tachibana M, et al. Dev Biol. 2012 Nov 15;371(2):146-55. doi: 10.1016/j.ydbio.2012.08.009. Epub 2012 Aug 23. Dev Biol. 2012. PMID: 22935618 Free PMC article. - Reprogramming somatic gene activity by fusion with pluripotent cells.
Do JT, Han DW, Schöler HR. Do JT, et al. Stem Cell Rev. 2006;2(4):257-64. doi: 10.1007/BF02698052. Stem Cell Rev. 2006. PMID: 17848712 Review. - Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the Burkitt's lymphoma line Daudi.
Gao Y, Smith PR, Karran L, Lu QL, Griffin BE. Gao Y, et al. J Virol. 1997 Jan;71(1):84-94. doi: 10.1128/JVI.71.1.84-94.1997. J Virol. 1997. PMID: 8985326 Free PMC article. - Characterization of the promoter region of the mouse Xist gene.
Pillet N, Bonny C, Schorderet DF. Pillet N, et al. Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12515-9. doi: 10.1073/pnas.92.26.12515. Proc Natl Acad Sci U S A. 1995. PMID: 8618932 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials