Mammalian homologs of Drosophila ELAV localized to a neuronal subset can bind in vitro to the 3' UTR of mRNA encoding the Id transcriptional repressor - PubMed (original) (raw)

Mammalian homologs of Drosophila ELAV localized to a neuronal subset can bind in vitro to the 3' UTR of mRNA encoding the Id transcriptional repressor

P H King et al. J Neurosci. 1994 Apr.

Abstract

Mammalian cDNAs encoding a rat (Rel-N1) and a human (Hel-N1) neuronal RNA-binding protein have been cloned and characterized with respect to tissue specificity, neuroanatomical localization, and RNA binding specificity. Both proteins are highly similar to the product of the Drosophila elav gene, which is expressed in all neurons of the fly and is required for development of the nervous system. However, in situ hybridization of rat tissues demonstrated more restricted expression of Rel-N1 mRNA within a subset of neurons of the hippocampus, cortex, and other regions of the gray matter, but not in glial cells or white matter. In vitro RNA binding experiments demonstrated that Hel-N1 can bind to the 3' untranslated region (3' UTR) of Id mRNA, a transcript that encodes a helix-loop-helix transcriptional repressor that is abundantly expressed in undifferentiated neural precursors. Sequences characterized for Hel-N1 binding were also abundantly present in the 3' UTR of the Drosophila extramacrochaetae mRNA, which encodes an Id homolog. Thus, we have identified a potential link between a neuronal 3' UTR RNA-binding protein and regulatory transcription factors involved in neural development. These findings are interpreted in light of recent studies in which mRNA 3' UTRs were found to be important for the regulation of cell growth and differentiation.

PubMed Disclaimer

Cited by

Alterations of RNA-binding protein found in neurons in Drosophila neurons and glia influence synaptic transmission and lifespan.
Lin WY, Liu CH, Cheng J, Liu HP. Lin WY, et al. Front Mol Neurosci. 2022 Nov 11;15:1006455. doi: 10.3389/fnmol.2022.1006455. eCollection 2022. Front Mol Neurosci. 2022. PMID: 36438188 Free PMC article.
RBP45 and RBP47, two oligouridylate-specific hnRNP-like proteins interacting with poly(A)+ RNA in nuclei of plant cells.
Lorković ZJ, Wieczorek Kirk DA, Klahre U, Hemmings-Mieszczak M, Filipowicz W. Lorković ZJ, et al. RNA. 2000 Nov;6(11):1610-24. doi: 10.1017/s1355838200001163. RNA. 2000. PMID: 11105760 Free PMC article.
HuR and TIA1/TIAL1 are involved in regulation of alternative splicing of SIRT1 pre-mRNA.
Zhao W, Zhao J, Hou M, Wang Y, Zhang Y, Zhao X, Zhang C, Guo D. Zhao W, et al. Int J Mol Sci. 2014 Feb 20;15(2):2946-58. doi: 10.3390/ijms15022946. Int J Mol Sci. 2014. PMID: 24566137 Free PMC article.
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Gao FB, Carson CC, Levine T, Keene JD. Gao FB, et al. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11207-11. doi: 10.1073/pnas.91.23.11207. Proc Natl Acad Sci U S A. 1994. PMID: 7972035 Free PMC article.
Coordinate repression of a trio of neuron-specific splicing events by the splicing regulator PTB.
Zhang L, Liu W, Grabowski PJ. Zhang L, et al. RNA. 1999 Jan;5(1):117-30. doi: 10.1017/s1355838299981530. RNA. 1999. PMID: 9917071 Free PMC article.

Mammalian homologs of Drosophila ELAV localized to a neuronal subset can bind in vitro to the 3' UTR of mRNA encoding the Id transcriptional repressor - PubMed (original) (raw)