The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor - PubMed (original) (raw)

Comparative Study

The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor

S Dou et al. Mol Cell Biol. 1994 May.

Abstract

We have identified a cellular protein, RBP-2N, a presumed recombinase, as a repressor of transcription. Inhibition of transcription by RBP-2N was dependent on its DNA recognition site and was demonstrated in vitro and in vivo. This repression appears to be general, as transcription mediated by SP1 and Gal4/VP16 was inhibited by RBP-2N. The protein was purified to near homogeneity from human cells on the basis of its binding to a site present in the promoter of the adenovirus pIX gene. The DNA recognition sequence is 5'-TGGGAAAGAA, which is markedly different from the recombination signal sequence originally identified as the target site for this protein. The sequence of the purified protein is 97% identical with that published for the mouse RBP-2N protein. The reported homolog in Drosophila is Suppressor of Hairless. RBP-2N binding sites are present in a number of cellular and viral promoters, so RBP-2N may have a general role in transcriptional repression.

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References

    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 - PubMed
    1. Genet Res. 1965 Jul;6:175-89 - PubMed
    1. Mol Cell Biol. 1990 Dec;10(12):6335-47 - PubMed
    1. Curr Opin Cell Biol. 1993 Jun;5(3):469-76 - PubMed
    1. Electrophoresis. 1990 Jul;11(7):537-53 - PubMed

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