Undermethylated transfer ribonucleic acid from a relaxed strain of Bacillus subtilis: construction of the strain and analysis of the transfer ribonucleic acid - PubMed (original) (raw)

Undermethylated transfer ribonucleic acid from a relaxed strain of Bacillus subtilis: construction of the strain and analysis of the transfer ribonucleic acid

N Keisel et al. J Bacteriol. 1976 Apr.

Abstract

A strain of Bacillus subtilis is described from which undermethylated transfer ribonucleic acid (tRNA) can be obtained. The tRNA's from a methionine-limited culture were compared with those from a control culture with respect to general nucleoside composition, methylated components, and amino acid acceptor activity. The undermethylated tRNA's had the normal amounts of the four major nucleosides, pseudouridine, and 5-methyluridine (ribothymidine), but were deficient in methylated nucleosides other than 5-methyluridine. These methyl-deficient nucleosides can be fully remethylated in the presence of the appropriate methylases. Since the majority of the work characterizing undermethylated tRNA's has been done using Escherichia coli, the work with B. subtilis presents some interesting comparisons and offers an alternative substrate for methylase studies.

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References

1. Genetics. 1947 Sep;32(5):505-25 - PubMed
1. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 - PubMed
1. Proc Natl Acad Sci U S A. 1945 Aug;31(8):215-9 - PubMed
1. Biochemistry. 1963 May-Jun;2:560-6 - PubMed
1. Methods Enzymol. 1974;29:502-10 - PubMed

Undermethylated transfer ribonucleic acid from a relaxed strain of Bacillus subtilis: construction of the strain and analysis of the transfer ribonucleic acid - PubMed (original) (raw)