The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation - PubMed (original) (raw)
Comparative Study
The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation
D Missiakas et al. EMBO J. 1994.
Abstract
We have identified and functionally characterized a new Escherichia coli gene, dsbC, whose product is involved in disulfide bond formation in the periplasmic space. It corresponds to a previously sequenced open reading frame mapping upstream of recJ with no previously assigned function. Null mutations in dsbC were obtained using a screen for dithiothreitol (DTT)-sensitive mutants and were shown to result in the accumulation of reduced forms of a variety of disulfide bond-containing periplasmic proteins. This defect could be rescued by the addition of either oxidized DTT or cystine or by multicopy expression of dsbA, a known periplasmic disulfide oxidase. The DsbC protein is synthesized as a precursor form of 25.5 kDa which is processed to a 23.3 kDa mature species located in the periplasmic space. The DsbC protein was overexpressed, purified to homogeneity and shown to catalyse the reduction of insulin in a DTT-dependent manner at levels comparable with those of purified DsbA. The replacement of either cysteine residue of the predicted active site, F-(X4)-C-G-Y-C, completely inactivates DsbC protein function. We have further shown that in vivo overexpression of DsbC can functionally substitute for a loss of DsbA function. Taken together, all of our results demonstrate that DsbC acts in vivo as a disulfide oxidase.
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References
- Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12058-62 - PubMed
- Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):1043-7 - PubMed
- Cell. 1991 Nov 1;67(3):581-9 - PubMed
- Mol Microbiol. 1992 Jul;6(14):1949-58 - PubMed
- J Biol Chem. 1993 Nov 25;268(33):24547-50 - PubMed
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