Autoantibodies from mixed cryoglobulinaemia patients bind glomerular antigens - PubMed (original) (raw)
Autoantibodies from mixed cryoglobulinaemia patients bind glomerular antigens
M P Dolcher et al. Clin Exp Immunol. 1994 May.
Abstract
Mixed cryoglobulinaemia (MC) is a disorder characterized by the presence of large amounts of cryoprecipitating IgM-IgG complexes. An immune complex glomerulonephritis develops in one third of all patients, but its occurrence does not seem related to the amount of cryoglobulins in the sera, nor to their complement-fixing ability. In this study we investigated the presence of IgG antibodies reactive with kidney antigens in 33 MC patients (11 with glomerulonephritis, 22 without renal involvement). A total glomerular extract was run on a 10% acrylamide gel, blotted to nitrocellulose and probed with the patients' sera. Sera from half of the patients without renal involvement reacted with several glomerular antigens whose molecular weight ranged between 200 and 29 kD. In the group with renal involvement, sera from 7/11 patients reacted with an antigen of 50 kD, which is also expressed in thymus, but not in the heart or liver. In a follow-up study of four patients with renal involvement, the amount of serum antibody specific for the 50-kD antigen fluctuated, either spontaneously or in response to therapy. These results show that antibodies specific for glomerular antigens are detectable in MC sera. The immune response against a 50-kD antigen expressed in the kidney and thymus seems to be restricted to a subset of MC patients with renal involvement. Circulating autoantibodies specific for glomerular antigens might contribute to the induction of glomerulonephritis in MC forming immune complexes in situ.
Similar articles
- Metabolic fate of monovalent and multivalent antibodies of Heymann nephritis following formation of surface immune complexes on glomerular epithelial cells.
Singh AK, Kasinath BS. Singh AK, et al. Clin Exp Immunol. 1993 Dec;94(3):403-11. doi: 10.1111/j.1365-2249.1993.tb08209.x. Clin Exp Immunol. 1993. PMID: 8252800 Free PMC article. - Type II mixed cryoglobulinaemia, hepatitis C virus infection, and glomerulonephritis.
Fornasieri A, D'Amico G. Fornasieri A, et al. Nephrol Dial Transplant. 1996;11 Suppl 4:25-30. doi: 10.1093/ndt/11.supp4.25. Nephrol Dial Transplant. 1996. PMID: 8918748 Review. - Mechanisms of glomerular injury: injury mediated by sensitized lymphocytes.
Bolton WK. Bolton WK. Semin Nephrol. 1991 May;11(3):285-93. Semin Nephrol. 1991. PMID: 2057643 Review. No abstract available.
Cited by
- Circulating anti-actin and anti-ATP synthase antibodies identify a sub-set of patients with idiopathic nephrotic syndrome.
Musante L, Candiano G, Bruschi M, Santucci L, Carnemolla B, Orecchia P, Giampuzzi M, Zennaro C, Sanna-Cherchi S, Carraro M, Oleggini R, Camussi G, Perfumo F, Ghiggeri GM. Musante L, et al. Clin Exp Immunol. 2005 Sep;141(3):491-9. doi: 10.1111/j.1365-2249.2005.02862.x. Clin Exp Immunol. 2005. PMID: 16045739 Free PMC article. - Antibody induced injury to podocytes with proteinuria and foot process swelling in a transgenic (T16) mouse.
Ebrahim H, Evans DJ. Ebrahim H, et al. Int J Exp Pathol. 1999 Apr;80(2):77-86. doi: 10.1046/j.1365-2613.1999.00098.x. Int J Exp Pathol. 1999. PMID: 10469262 Free PMC article. - Cryoglobulins and cryoglobulinemia. Diagnostic and therapeutic considerations.
Della Rossa A, Trevisani G, Bombardieri S. Della Rossa A, et al. Clin Rev Allergy Immunol. 1998 Fall;16(3):249-64. doi: 10.1007/BF02737635. Clin Rev Allergy Immunol. 1998. PMID: 9773252 Review. No abstract available.
References
- J Immunol. 1987 Jun 1;138(11):3785-92 - PubMed
- Eur J Clin Invest. 1993 Jul;23(7):399-405 - PubMed
- J Cell Biol. 1989 Mar;108(3):1139-48 - PubMed
- J Immunol Methods. 1989 May 12;119(2):153-87 - PubMed
- J Immunol. 1990 Dec 15;145(12):4089-93 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources