Absence of a Ku-like DNA end binding activity in the xrs double-strand DNA repair-deficient mutant - PubMed (original) (raw)

. 1994 Jun 10;269(23):15981-4.

Affiliations

• PMID: 8206892

Free article

Absence of a Ku-like DNA end binding activity in the xrs double-strand DNA repair-deficient mutant

R C Getts et al. J Biol Chem. 1994.

Free article

Abstract

Double-strand DNA break repair is important in maintaining the genetic integrity of the genome. Using a mobility shift assay, we find that a protein, or complex of proteins, that is present in mammalian and yeast cells binds to the ends of double-strand DNA and renders the ends resistant to exonuclease digestion. Additionally, a mammalian DNA double-strand repair-deficient mutant, xrs, has no observable DNA end binding activity, while a revertant cell has wild-type activity. In addition, mobility supershift assays using monoclonal antibodies to the human Ku antigen (M(r) 70,000 subunit) reveal that one of the proteins of this end binding activity may be the Ku antigen or a protein with similar antigenic determinants. These observations suggest that this DNA end-binding protein may function in DNA repair.

PubMed Disclaimer

Similar articles

• Analysis of the DNA replication competence of the xrs-5 mutant cells defective in Ku86.
  Matheos D, Novac O, Price GB, Zannis-Hadjopoulos M. Matheos D, et al. J Cell Sci. 2003 Jan 1;116(Pt 1):111-24. doi: 10.1242/jcs.00156. J Cell Sci. 2003. PMID: 12456721
• DNA-PK-dependent binding of DNA ends to plasmids containing nuclear matrix attachment region DNA sequences: evidence for assembly of a repair complex.
  Mauldin SK, Getts RC, Liu W, Stamato TD. Mauldin SK, et al. Nucleic Acids Res. 2002 Sep 15;30(18):4075-87. doi: 10.1093/nar/gkf529. Nucleic Acids Res. 2002. PMID: 12235392 Free PMC article.
• Disruption of DNA-PK in Ku80 mutant xrs-6 and the implications in DNA double-strand break repair.
  Chen F, Peterson SR, Story MD, Chen DJ. Chen F, et al. Mutat Res. 1996 Jan 2;362(1):9-19. doi: 10.1016/0921-8777(95)00026-7. Mutat Res. 1996. PMID: 8538653
• DNA double-strand break repair.
  Featherstone C, Jackson SP. Featherstone C, et al. Curr Biol. 1999 Oct 21;9(20):R759-61. doi: 10.1016/S0960-9822(00)80005-6. Curr Biol. 1999. PMID: 10531043 Review. No abstract available.
• DNA double-strand break repair from head to tail.
  Hopfner KP, Putnam CD, Tainer JA. Hopfner KP, et al. Curr Opin Struct Biol. 2002 Feb;12(1):115-22. doi: 10.1016/s0959-440x(02)00297-x. Curr Opin Struct Biol. 2002. PMID: 11839498 Review.

Cited by

• Nuclear Actin Dynamics in Gene Expression, DNA Repair, and Cancer.
  Huang Y, Zhang S, Park JI. Huang Y, et al. Results Probl Cell Differ. 2022;70:625-663. doi: 10.1007/978-3-031-06573-6_23. Results Probl Cell Differ. 2022. PMID: 36348125 Free PMC article.
• What Does the History of Research on the Repair of DNA Double-Strand Breaks Tell Us?-A Comprehensive Review of Human Radiosensitivity.
  Berthel E, Ferlazzo ML, Devic C, Bourguignon M, Foray N. Berthel E, et al. Int J Mol Sci. 2019 Oct 26;20(21):5339. doi: 10.3390/ijms20215339. Int J Mol Sci. 2019. PMID: 31717816 Free PMC article. Review.
• The arginine methyltransferase PRMT5 and PRMT1 distinctly regulate the degradation of anti-apoptotic protein CFLARL in human lung cancer cells.
  Li M, An W, Xu L, Lin Y, Su L, Liu X. Li M, et al. J Exp Clin Cancer Res. 2019 Feb 8;38(1):64. doi: 10.1186/s13046-019-1064-8. J Exp Clin Cancer Res. 2019. PMID: 30736843 Free PMC article.
• Yeast-based assays for characterization of the functional effects of single nucleotide polymorphisms in human DNA repair genes.
  Kim C, Yang J, Jeong SH, Kim H, Park GH, Shin HB, Ro M, Kim KY, Park Y, Kim KP, Kwack K. Kim C, et al. PLoS One. 2018 Mar 9;13(3):e0193823. doi: 10.1371/journal.pone.0193823. eCollection 2018. PLoS One. 2018. PMID: 29522548 Free PMC article.
• The role of DNA-PK in aging and energy metabolism.
  Chung JH. Chung JH. FEBS J. 2018 Jun;285(11):1959-1972. doi: 10.1111/febs.14410. Epub 2018 Mar 12. FEBS J. 2018. PMID: 29453899 Free PMC article. Review.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • Saccharomyces Genome Database

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal