Vav binds to several SH2/SH3 containing proteins in activated lymphocytes - PubMed (original) (raw)
Affiliations
- PMID: 8208538
Vav binds to several SH2/SH3 containing proteins in activated lymphocytes
F Ramos-Morales et al. Oncogene. 1994 Jul.
Abstract
In T lymphocytes, several proteins are rapidly phosphorylated on tyrosine after stimulation. In this study we examine the ability of tyrosine phosphorylated proteins from Jurkat T cells stimulated by CD2 or T cell receptor-CD3 to interact with the src homology 2 or src homology 3 domains from eight different proteins involved in signal transduction in lymphocytes: Vav, Shc, Nck, phosphatidylinositol-3-kinase, phospholipase C-gamma 1, Ras-GTPase activating protein, c-Crk and Grb2. Our data show that different SH2 domains have distinct patterns of binding to phosphotyrosine containing proteins. We show that Vav, a protein expressed only in hematopoietic cells that may have guanine nucleotide releasing factor activity, is able to interact with certain SH2-containing proteins depending on its tyrosine phosphorylation and with Grb2 in a manner independent of phosphorylation on tyrosine. Coimmunoprecipitation experiments support the idea of a trimolecular complex Shc-Grb2-Vav in vivo. These data suggest a central role played by Vav and provide insight in the complexity and specificity of protein-protein interactions in the signaling events in lymphocytes.
Similar articles
- P56lck: a transducing protein that binds to SH2 containing proteins and to phosphotyrosine containing proteins.
Ramos-Morales F, Douté M, Fischer S. Ramos-Morales F, et al. Cell Mol Biol (Noisy-le-grand). 1994 Jul;40(5):695-700. Cell Mol Biol (Noisy-le-grand). 1994. PMID: 7526919 - The proline-rich region of Vav binds to Grb2 and Grb3-3.
Ramos-Morales F, Romero F, Schweighoffer F, Bismuth G, Camonis J, Tortolero M, Fischer S. Ramos-Morales F, et al. Oncogene. 1995 Oct 19;11(8):1665-9. Oncogene. 1995. PMID: 7478592 - Tyrosine phosphorylation of vav proto-oncogene product containing SH2 domain and transcription factor motifs.
Margolis B, Hu P, Katzav S, Li W, Oliver JM, Ullrich A, Weiss A, Schlessinger J. Margolis B, et al. Nature. 1992 Mar 5;356(6364):71-4. doi: 10.1038/356071a0. Nature. 1992. PMID: 1531699 - SH2 and SH3-containing adaptor proteins: redundant or independent mediators of intracellular signal transduction.
Birge RB, Knudsen BS, Besser D, Hanafusa H. Birge RB, et al. Genes Cells. 1996 Jul;1(7):595-613. doi: 10.1046/j.1365-2443.1996.00258.x. Genes Cells. 1996. PMID: 9078388 Review. - [Lymphocytes: what is "Vav"].
Charvet C, Deckert M. Charvet C, et al. Med Sci (Paris). 2003 Feb;19(2):217-22. doi: 10.1051/medsci/2003192217. Med Sci (Paris). 2003. PMID: 12836616 Review. French.
Cited by
- The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH(2)-terminal kinase 1.
Arudchandran R, Brown MJ, Peirce MJ, Song JS, Zhang J, Siraganian RP, Blank U, Rivera J. Arudchandran R, et al. J Exp Med. 2000 Jan 3;191(1):47-60. doi: 10.1084/jem.191.1.47. J Exp Med. 2000. PMID: 10620604 Free PMC article. - Vav1 Regulates T-Cell Activation through a Feedback Mechanism and Crosstalk between the T-Cell Receptor and CD28.
Helou YA, Petrashen AP, Salomon AR. Helou YA, et al. J Proteome Res. 2015 Jul 2;14(7):2963-75. doi: 10.1021/acs.jproteome.5b00340. Epub 2015 Jun 16. J Proteome Res. 2015. PMID: 26043137 Free PMC article. - Tyrosine phosphorylation of the proto-oncogene product Vav and its association with the adapter Grb2/Ash in a human leukemia cell line UT-7.
Hanazono Y, Sasaki K, Odai H, Mimura T, Mitani K, Yazaki Y, Hirai H. Hanazono Y, et al. Jpn J Cancer Res. 1995 Apr;86(4):336-41. doi: 10.1111/j.1349-7006.1995.tb03061.x. Jpn J Cancer Res. 1995. PMID: 7539782 Free PMC article. - Vav1 transduces T cell receptor signals to the activation of phospholipase C-gamma1 via phosphoinositide 3-kinase-dependent and -independent pathways.
Reynolds LF, Smyth LA, Norton T, Freshney N, Downward J, Kioussis D, Tybulewicz VL. Reynolds LF, et al. J Exp Med. 2002 May 6;195(9):1103-14. doi: 10.1084/jem.20011663. J Exp Med. 2002. PMID: 11994416 Free PMC article. - p95vav associates with the nuclear protein Ku-70.
Romero F, Dargemont C, Pozo F, Reeves WH, Camonis J, Gisselbrecht S, Fischer S. Romero F, et al. Mol Cell Biol. 1996 Jan;16(1):37-44. doi: 10.1128/MCB.16.1.37. Mol Cell Biol. 1996. PMID: 8524317 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials
Miscellaneous