Molecular cloning of a human small intestinal apolipoprotein B mRNA editing protein - PubMed (original) (raw)

Molecular cloning of a human small intestinal apolipoprotein B mRNA editing protein

C Hadjiagapiou et al. Nucleic Acids Res. 1994.

Free PMC article

Abstract

Mammalian small intestinal apolipoprotein B (apo B) mRNA undergoes posttranscriptional cytidine deamination with the production of an in frame stop codon and the translation of apo B48. We have isolated a cDNA from human jejunum which mediates in vitro editing of a synthetic apo B RNA template upon complementation with chicken intestinal S100 extracts. The cDNA specifies a 236 residue protein which is 69% identical to the apo B mRNA editing protein (REPR) cloned from rat small intestine [Teng, B., Burant, C. F. and Davidson, N. O. (1993) Science 260, 1816-1819] and which, by analogy, is referred to as HEPR. HEPR does not contain the carboxyl-terminus leucine zipper motif identified in REPR but contains consensus phosphorylation sites as well as the conserved histidine and both cysteine residues identified as a Zn2+ binding motif in other cytidine deaminases. The distribution of HEPR mRNA was predominantly confined to the adult small intestine with lower levels detectable by reverse-transcription polymerase chain reaction amplification in the stomach, colon and testis. These differences in the structure and distribution of the human as compared to the rat apo B mRNA editing protein suggest an important evolutionary adaptation in the mechanisms restricting apo B48 production to the small intestine.

PubMed Disclaimer

Cited by

C-->U editing of neurofibromatosis 1 mRNA occurs in tumors that express both the type II transcript and apobec-1, the catalytic subunit of the apolipoprotein B mRNA-editing enzyme.
Mukhopadhyay D, Anant S, Lee RM, Kennedy S, Viskochil D, Davidson NO. Mukhopadhyay D, et al. Am J Hum Genet. 2002 Jan;70(1):38-50. doi: 10.1086/337952. Epub 2001 Nov 27. Am J Hum Genet. 2002. PMID: 11727199 Free PMC article.
APOBEC1-mediated editing and attenuation of herpes simplex virus 1 DNA indicate that neurons have an antiviral role during herpes simplex encephalitis.
Gee P, Ando Y, Kitayama H, Yamamoto SP, Kanemura Y, Ebina H, Kawaguchi Y, Koyanagi Y. Gee P, et al. J Virol. 2011 Oct;85(19):9726-36. doi: 10.1128/JVI.05288-11. Epub 2011 Jul 20. J Virol. 2011. PMID: 21775448 Free PMC article.
APOBEC-1-mediated RNA editing.
Blanc V, Davidson NO. Blanc V, et al. Wiley Interdiscip Rev Syst Biol Med. 2010 Sep-Oct;2(5):594-602. doi: 10.1002/wsbm.82. Wiley Interdiscip Rev Syst Biol Med. 2010. PMID: 20836050 Free PMC article. Review.
Role of the gut in lipid homeostasis.
Abumrad NA, Davidson NO. Abumrad NA, et al. Physiol Rev. 2012 Jul;92(3):1061-85. doi: 10.1152/physrev.00019.2011. Physiol Rev. 2012. PMID: 22811425 Free PMC article. Review.

References

1. Biochem Biophys Res Commun. 1993 Sep 30;195(3):1204-10 - PubMed
1. J Biol Chem. 1993 Oct 5;268(28):20709-12 - PubMed
1. J Lipid Res. 1994 Feb;35(2):340-50 - PubMed
1. J Biol Chem. 1993 Feb 5;268(4):2288-91 - PubMed
1. Cell. 1987 Sep 11;50(6):831-40 - PubMed

Molecular cloning of a human small intestinal apolipoprotein B mRNA editing protein - PubMed (original) (raw)