Importance of exocyclic base functional groups of central core guanosines for hammerhead ribozyme activity - PubMed (original) (raw)
. 1993 Nov 2;32(43):11658-68.
doi: 10.1021/bi00094a023.
Affiliations
- PMID: 8218233
- DOI: 10.1021/bi00094a023
Importance of exocyclic base functional groups of central core guanosines for hammerhead ribozyme activity
T Tuschl et al. Biochemistry. 1993.
Erratum in
- Biochemistry 1994 Jan 25;33(3):848
Abstract
The three guanosines of the central core of a hammerhead ribozyme were replaced by 2-aminopurine ribonucleoside, xanthosine, isoguanosine, inosine, and deoxyguanosine. These analogues were incorporated by automated solid-phase synthesis, with the exception of isoguanosine. This was introduced by ligating a donor, which carried the isoguanosine at its 5'-end, and an acceptor oligoribonucleotide by a T4 DNA ligase-catalyzed reaction. Most of these modifications lowered the rate constant of cleavage by the hammerhead ribozyme drastically. Inspection of the possible hydrogen-bonding interactions disturbed by these modifications suggests that there is no G12A9 or A13G8 mismatched base pair in the central region. Increasing the Mg2+ concentration from 10 to 50 mM did not enhance these rates appreciably. This makes it improbable that the guanosines, including their 2'-hydroxyl groups, are involved in the binding of the catalytically active Mg2+. Transition-state destabilizing energies of 0.6-4.7 kcal mol-1 suggest that essentially all guanosines are involved in a hydrogen-bonding network.
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