Excitation energy transfer in DNA: duplex melting and transfer from normal bases to 2-aminopurine - PubMed (original) (raw)
. 1993 Nov 16;32(45):12090-5.
doi: 10.1021/bi00096a020.
Affiliations
- PMID: 8218287
- DOI: 10.1021/bi00096a020
Excitation energy transfer in DNA: duplex melting and transfer from normal bases to 2-aminopurine
T M Nordlund et al. Biochemistry. 1993.
Abstract
Absorption and fluorescence excitation and emission spectra of the B DNA duplex decamer d[CTGA(2AP)TTCAG]2, where emission from the 2AP (2-aminopurine) base dominates, have been measured as a function of temperature. A low-temperature excitation band in the 260-270-nm region disappears near the duplex melting temperature, Tm = 27 degrees C, but then reappears at higher temperatures. Singlet-singlet energy transfer thus occurs between the normal DNA bases and the 2AP base in the B-helical conformation and to a lesser extent in the structurally-mobile melted conformation. The measured efficiency of transfer is 4-5% at 4 degrees C, near 0 at 30 degrees C, and rises again to 1% at 48 degrees C. Nearest-neighbor-only singlet transfer is likely. Such transfer does not offer a likely explanation for UV damage distributions in DNA.
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