Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis - PubMed (original) (raw)
Comparative Study
. 1993 Nov 5;268(31):23524-30.
Affiliations
- PMID: 8226881
Free article
Comparative Study
Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis
K Sakumi et al. J Biol Chem. 1993.
Free article
Abstract
8-Oxoguanine (8-oxo-7, 8-dihydroguanine) is produced in DNA, as well as in nucleotide pools of cells, by active oxygen species normally formed during cellular metabolic processes. 8-Oxoguanine nucleotide can pair with cytosine and adenine nucleotides at almost equal efficiencies, and transversion mutation ensues. Human cells contain enzyme activity, which hydrolyzes 8-oxo-dGTP to 8-oxo-dGMP, and this enzyme is responsible for preventing misincorporation of 8-oxoguanine into DNA. We purified this particular human enzyme to physical homogeneity and determined a partial amino acid sequence. We then cloned the cDNA for human 8-oxo-dGTPase and examined its nucleotide sequence. The human protein comprises 156 amino acid residues and has some sequence homology with the Escherichia coli MutT protein, which has a distinct 8-oxo-dGTPase activity. When the human cDNA was expressed in E. coli mutT- mutant cells, there was a significant amount of 8-oxo-dGTPase activity. In such cells, the frequency of spontaneous mutation was greatly reduced. We propose that the human 8-oxo-dGTPase protects genetic information from the untoward effects of endogenous oxygen radicals.
Similar articles
- Mouse MTH1 protein with 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphatase activity that prevents transversion mutation. cDNA cloning and tissue distribution.
Kakuma T, Nishida J, Tsuzuki T, Sekiguchi M. Kakuma T, et al. J Biol Chem. 1995 Oct 27;270(43):25942-8. doi: 10.1074/jbc.270.43.25942. J Biol Chem. 1995. PMID: 7592783 - cDNA and genomic sequences for rat 8-oxo-dGTPase that prevents occurrence of spontaneous mutations due to oxidation of guanine nucleotides.
Cai JP, Kakuma T, Tsuzuki T, Sekiguchi M. Cai JP, et al. Carcinogenesis. 1995 Oct;16(10):2343-50. doi: 10.1093/carcin/16.10.2343. Carcinogenesis. 1995. PMID: 7586133 - Genomic structure and chromosome location of the human mutT homologue gene MTH1 encoding 8-oxo-dGTPase for prevention of A:T to C:G transversion.
Furuichi M, Yoshida MC, Oda H, Tajiri T, Nakabeppu Y, Tsuzuki T, Sekiguchi M. Furuichi M, et al. Genomics. 1994 Dec;24(3):485-90. doi: 10.1006/geno.1994.1657. Genomics. 1994. PMID: 7713500 - Oxidative nucleotide damage: consequences and prevention.
Sekiguchi M, Tsuzuki T. Sekiguchi M, et al. Oncogene. 2002 Dec 16;21(58):8895-904. doi: 10.1038/sj.onc.1206023. Oncogene. 2002. PMID: 12483507 Review. - MutT-related error avoidance mechanism for DNA synthesis.
Sekiguchi M. Sekiguchi M. Genes Cells. 1996 Feb;1(2):139-45. doi: 10.1046/j.1365-2443.1996.d01-232.x. Genes Cells. 1996. PMID: 9140059 Review.
Cited by
- Relationships among smoking, oxidative stress, inflammation, macromolecular damage, and cancer.
Caliri AW, Tommasi S, Besaratinia A. Caliri AW, et al. Mutat Res Rev Mutat Res. 2021 Jan-Jun;787:108365. doi: 10.1016/j.mrrev.2021.108365. Epub 2021 Jan 11. Mutat Res Rev Mutat Res. 2021. PMID: 34083039 Free PMC article. Review. - Molecular devices for high fidelity of DNA replication and gene expression.
Sekiguchi M. Sekiguchi M. Proc Jpn Acad Ser B Phys Biol Sci. 2006 Dec;82(8):278-96. doi: 10.2183/pjab.82.278. Epub 2006 Dec 2. Proc Jpn Acad Ser B Phys Biol Sci. 2006. PMID: 25792791 Free PMC article. Review. - Development of bimolecular fluorescence complementation using Dronpa for visualization of protein-protein interactions in cells.
Lee YR, Park JH, Hahm SH, Kang LW, Chung JH, Nam KH, Hwang KY, Kwon IC, Han YS. Lee YR, et al. Mol Imaging Biol. 2010 Oct;12(5):468-78. doi: 10.1007/s11307-010-0312-2. Mol Imaging Biol. 2010. PMID: 20373040 - Purification and characterization of a mammalian homolog of Escherichia coli MutY mismatch repair protein from calf liver mitochondria.
Parker A, Gu Y, Lu AL. Parker A, et al. Nucleic Acids Res. 2000 Sep 1;28(17):3206-15. doi: 10.1093/nar/28.17.3206. Nucleic Acids Res. 2000. PMID: 10954587 Free PMC article. - Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase.
Tsuzuki T, Egashira A, Igarashi H, Iwakuma T, Nakatsuru Y, Tominaga Y, Kawate H, Nakao K, Nakamura K, Ide F, Kura S, Nakabeppu Y, Katsuki M, Ishikawa T, Sekiguchi M. Tsuzuki T, et al. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11456-61. doi: 10.1073/pnas.191086798. Proc Natl Acad Sci U S A. 2001. PMID: 11572992 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases