Yeast origin recognition complex is involved in DNA replication and transcriptional silencing - PubMed (original) (raw)

. 1993 Nov 4;366(6450):87-9.

doi: 10.1038/366087a0.

Affiliations

• PMID: 8232543
• DOI: 10.1038/366087a0

Yeast origin recognition complex is involved in DNA replication and transcriptional silencing

G Micklem et al. Nature. 1993.

Abstract

The HMR E silencer represses transcription of silent mating-type genes in the budding yeast Saccharomyces cerevisiae and contains three redundant regulatory elements A, E and B (ref. 1). The A element contains the 11 base pair consensus sequence that is essential for the firing of DNA replication origins. A multisubunit protein called the origin recognition complex (ORC) binds specifically to this consensus sequence within yeast origins in vitro and in vivo. We isolated mutants in A element-mediated silencing and report here that one of the genes we identified, RRR1, encodes ORC2, the 72K subunit of ORC. RRR1/ORC2 is an essential gene, but the rrr1-316 allele, which is viable, is defective in the replication of nuclear DNA and the maintenance of the 2-microns episomal DNA. This is, to our knowledge, the first genetic evidence that ORC is involved in DNA replication and silencing.

PubMed Disclaimer

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • BioCyc
  • Saccharomyces Genome Database