Cloning the RAD51 homologue of Schizosaccharomyces pombe - PubMed (original) (raw)

Comparative Study

. 1993 Sep 25;21(19):4586-91.

doi: 10.1093/nar/21.19.4586.

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Free PMC article

Comparative Study

Cloning the RAD51 homologue of Schizosaccharomyces pombe

D F Muris et al. Nucleic Acids Res. 1993.

Free PMC article

Abstract

The RAD51 gene of Saccharomyces cerevisiae encodes a RecA like protein, which is involved in the recombinational repair of double strand breaks. We have isolated the RAD51 homologue, rhp51+, of the distantly related yeast strain Schizosaccharomyces pombe by heterologous hybridization. DNA sequence analysis of the rhp51+ gene revealed an open reading frame of 365 amino acids. Comparison of the amino acid sequences of RAD51 and rhp51+ showed a high level of conservation: 69% identical amino acids. There are two Mlul sites in the upstream region which may be associated with cell cycle regulation of the rhp51+ gene. The rhp51+ null allele, constructed by disruption of the coding region, is extremely sensitive to X-rays, indicating that the rhp51+ gene, like RAD51, is also involved in the repair of X-ray damage. The structural and functional homology between rhp51+ and RAD51 suggests evolutionary conservation of certain steps in the recombinational repair pathway.

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References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Semin Cancer Biol. 1993 Apr;4(2):73-83 - PubMed
    1. Nucleic Acids Res. 1983 May 11;11(9):2871-80 - PubMed
    1. Gene. 1985;33(1):103-19 - PubMed
    1. Nature. 1985 Nov 7-13;318(6041):78-80 - PubMed

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