Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes - PubMed (original) (raw)

Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes

A Tassanakajon et al. Trans R Soc Trop Med Hyg. 1993 May-Jun.

Abstract

The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections.

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