Cloning and characterization of alpha PS1, a novel Drosophila melanogaster integrin - PubMed (original) (raw)
Comparative Study
Cloning and characterization of alpha PS1, a novel Drosophila melanogaster integrin
M Wehrli et al. Mech Dev. 1993 Sep.
Abstract
The Drosophila position-specific integrins (PS integrins or PS antigens) comprise two heterodimeric complexes, alpha PS1 beta PS and alpha PS2 beta PS. With the cloning of alpha PS1 described here, we complete the characterization of the primary structure of the three PS integrin subunits. We have purified the alpha PS1 subunit, obtained peptide sequence and isolated genomic and cDNA clones. The encoded alpha PS1 protein contains the cysteine pattern of the cleaved alpha integrins, three putative metal binding domains and shows the other characteristic features of alpha integrins. Regions of sequence variation indicate that alpha PS1 is distinct from all other alpha chains. The transcript analysis shows that the patterns of both alpha PS1 mRNA and protein expression are the same, suggesting that the gene is controlled transcriptionally. We compare the gene structures of the Drosophila alpha PS1, alpha PS2, the human alpha IIb and alpha X (p150,95) and the C. elegans F54G8.3 integrins. We find several positions and phases of introns conserved which, supported by conservation also in the amino acid sequence, indicates that they all derive from a common ancestral gene.
Similar articles
- Null mutations in the alpha PS2 and beta PS integrin subunit genes have distinct phenotypes.
Brown NH. Brown NH. Development. 1994 May;120(5):1221-31. doi: 10.1242/dev.120.5.1221. Development. 1994. PMID: 8026331 - Nonequivalent requirements for PS1 and PS2 integrin at cell attachments in Drosophila: genetic analysis of the alpha PS1 integrin subunit.
Brower DL, Bunch TA, Mukai L, Adamson TE, Wehrli M, Lam S, Friedlander E, Roote CE, Zusman S. Brower DL, et al. Development. 1995 May;121(5):1311-20. doi: 10.1242/dev.121.5.1311. Development. 1995. PMID: 7789263 - The Drosophila melanogaster flightless-I gene involved in gastrulation and muscle degeneration encodes gelsolin-like and leucine-rich repeat domains and is conserved in Caenorhabditis elegans and humans.
Campbell HD, Schimansky T, Claudianos C, Ozsarac N, Kasprzak AB, Cotsell JN, Young IG, de Couet HG, Miklos GL. Campbell HD, et al. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11386-90. doi: 10.1073/pnas.90.23.11386. Proc Natl Acad Sci U S A. 1993. PMID: 8248259 Free PMC article. - The PS integrins are required for a regulatory event during Drosophila wing morphogenesis.
Brabant MC, Fristrom D, Bunch TA, Baker SE, Brower DL. Brabant MC, et al. Ann N Y Acad Sci. 1998 Oct 23;857:99-109. doi: 10.1111/j.1749-6632.1998.tb10110.x. Ann N Y Acad Sci. 1998. PMID: 9917835 Review. - Role of the PS integrins in Drosophila development.
Brower DL, Brabant MC, Bunch TA. Brower DL, et al. Immunol Cell Biol. 1995 Dec;73(6):558-64. doi: 10.1038/icb.1995.89. Immunol Cell Biol. 1995. PMID: 8713479 Review.
Cited by
- Chitin deacetylases are necessary for insect femur muscle attachment and mobility.
Mun S, Noh MY, Geisbrecht ER, Kramer KJ, Muthukrishnan S, Arakane Y. Mun S, et al. Proc Natl Acad Sci U S A. 2022 Jun 14;119(24):e2120853119. doi: 10.1073/pnas.2120853119. Epub 2022 Jun 8. Proc Natl Acad Sci U S A. 2022. PMID: 35675426 Free PMC article. - Integrins Have Cell-Type-Specific Roles in the Development of Motor Neuron Connectivity.
Oliver D, Norman E, Bates H, Avard R, Rettler M, Bénard CY, Francis MM, Lemons ML. Oliver D, et al. J Dev Biol. 2019 Aug 27;7(3):17. doi: 10.3390/jdb7030017. J Dev Biol. 2019. PMID: 31461926 Free PMC article. - Drosophila canopy b is a cochaperone of glycoprotein 93.
Morales C, Li Z. Morales C, et al. J Biol Chem. 2017 Apr 21;292(16):6657-6666. doi: 10.1074/jbc.M116.755538. Epub 2017 Mar 8. J Biol Chem. 2017. PMID: 28275054 Free PMC article. - Netrins and Frazzled/DCC promote the migration and mesenchymal to epithelial transition of Drosophila midgut cells.
Pert M, Gan M, Saint R, Murray MJ. Pert M, et al. Biol Open. 2015 Jan 23;4(2):233-43. doi: 10.1242/bio.201410827. Biol Open. 2015. PMID: 25617422 Free PMC article. - PS integrins and laminins: key regulators of cell migration during Drosophila embryogenesis.
Urbano JM, Domínguez-Giménez P, Estrada B, Martín-Bermudo MD. Urbano JM, et al. PLoS One. 2011;6(9):e23893. doi: 10.1371/journal.pone.0023893. Epub 2011 Sep 16. PLoS One. 2011. PMID: 21949686 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases