A second pathway for regeneration of adult exocrine and endocrine pancreas. A possible recapitulation of embryonic development - PubMed (original) (raw)
A second pathway for regeneration of adult exocrine and endocrine pancreas. A possible recapitulation of embryonic development
S Bonner-Weir et al. Diabetes. 1993 Dec.
Abstract
Substantial regeneration of both the endocrine and exocrine pancreas occurs after a 90% partial pancreatectomy in the young adult rat. We have reported previously that replication of preexisting islet and exocrine cells is enhanced 3- to 4-fold. Here, we report a second pathway of regeneration, that of proliferation and differentiation of precursor cells in the ductal epithelium. As shown with in vivo pulse labeling using 5-bromo-2'-deoxyuridine, an expansion of the ductal epithelium occurs. Proliferation is seen first in the common pancreatic duct and sequentially in smaller ducts of the ductal tree as focal areas of proliferation small ductules form. By 60 h after pancreatectomy, only these focal areas show heavy 5-bromo-2'-deoxyuridine staining. These proliferating ductules comprise 12.8% of the pancreatic volume at 3 days after pancreatectomy but are uncommon at 7 days after pancreatectomy. Coincident with the appearance and disappearance of these regions was a 3.5-fold increased growth of the pancreatic remnant compared with its equivalent of sham animals. These small ductules differentiate into new pancreatic islets and exocrine tissue, forming new lobules of pancreas that are indistinguishable from the preexisting ones. This second pathway of rapid regeneration recapitulates embryonic development in its pattern of ductal proliferation and subsequent differentiation. Furthermore, these studies provide evidence of the presence of precursor/stem cells in the adult pancreas.
Similar articles
- Activation of pancreatic-duct-derived progenitor cells during pancreas regeneration in adult rats.
Li WC, Rukstalis JM, Nishimura W, Tchipashvili V, Habener JF, Sharma A, Bonner-Weir S. Li WC, et al. J Cell Sci. 2010 Aug 15;123(Pt 16):2792-802. doi: 10.1242/jcs.065268. Epub 2010 Jul 27. J Cell Sci. 2010. PMID: 20663919 Free PMC article. - Differentiation and proliferation of endocrine cells in the regenerating rat pancreas after 90% pancreatectomy.
Hayashi KY, Tamaki H, Handa K, Takahashi T, Kakita A, Yamashina S. Hayashi KY, et al. Arch Histol Cytol. 2003 May;66(2):163-74. doi: 10.1679/aohc.66.163. Arch Histol Cytol. 2003. PMID: 12846556 - The homeodomain protein IDX-1 increases after an early burst of proliferation during pancreatic regeneration.
Sharma A, Zangen DH, Reitz P, Taneja M, Lissauer ME, Miller CP, Weir GC, Habener JF, Bonner-Weir S. Sharma A, et al. Diabetes. 1999 Mar;48(3):507-13. doi: 10.2337/diabetes.48.3.507. Diabetes. 1999. PMID: 10078550 - Proliferation and differentiation of adult rat pancreatic exocrine cells.
Rooman I. Rooman I. Verh K Acad Geneeskd Belg. 2003;65(2):117-25. Verh K Acad Geneeskd Belg. 2003. PMID: 12870182 Review. No abstract available. - Can pancreatic duct-derived progenitors be a source of islet regeneration?
Xia B, Zhan XR, Yi R, Yang B. Xia B, et al. Biochem Biophys Res Commun. 2009 Jun 12;383(4):383-5. doi: 10.1016/j.bbrc.2009.03.114. Epub 2009 Mar 24. Biochem Biophys Res Commun. 2009. PMID: 19324022 Review.
Cited by
- β-Cell Regeneration Is Driven by Pancreatic Plasticity.
Holguín-Horcajo A, Sancho R, Rovira M. Holguín-Horcajo A, et al. Adv Anat Embryol Cell Biol. 2024;239:91-115. doi: 10.1007/978-3-031-62232-8_4. Adv Anat Embryol Cell Biol. 2024. PMID: 39283483 Review. - Development, regeneration, and physiological expansion of functional β-cells: Cellular sources and regulators.
Chernysheva МB, Ruchko ЕS, Karimova МV, Vorotelyak ЕA, Vasiliev АV. Chernysheva МB, et al. Front Cell Dev Biol. 2024 Jul 9;12:1424278. doi: 10.3389/fcell.2024.1424278. eCollection 2024. Front Cell Dev Biol. 2024. PMID: 39045459 Free PMC article. Review. - EZH2 inhibitors promote β-like cell regeneration in young and adult type 1 diabetes donors.
Al-Hasani K, Marikar SN, Kaipananickal H, Maxwell S, Okabe J, Khurana I, Karagiannis T, Liang JJ, Mariana L, Loudovaris T, Kay T, El-Osta A. Al-Hasani K, et al. Signal Transduct Target Ther. 2024 Jan 1;9(1):2. doi: 10.1038/s41392-023-01707-x. Signal Transduct Target Ther. 2024. PMID: 38161208 Free PMC article. - Pancreatic regenerative potential of manuka honey evidenced through pancreatic histology and levels of transcription factors in diabetic rat model.
Iftikhar A, Nausheen R, Khurshid M, Iqbal RK, Muzaffar H, Malik A, Ali Khan A, Batool F, Akhtar S, Yasin A, Anwar H. Iftikhar A, et al. Heliyon. 2023 Sep 9;9(9):e20017. doi: 10.1016/j.heliyon.2023.e20017. eCollection 2023 Sep. Heliyon. 2023. PMID: 37809953 Free PMC article. - Emerging therapeutic options in the management of diabetes: recent trends, challenges and future directions.
Ansari MA, Chauhan W, Shoaib S, Alyahya SA, Ali M, Ashraf H, Alomary MN, Al-Suhaimi EA. Ansari MA, et al. Int J Obes (Lond). 2023 Dec;47(12):1179-1199. doi: 10.1038/s41366-023-01369-3. Epub 2023 Sep 11. Int J Obes (Lond). 2023. PMID: 37696926 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials