Molecular characterization of a maize B chromosome centric sequence - PubMed (original) (raw)

Molecular characterization of a maize B chromosome centric sequence

M R Alfenito et al. Genetics. 1993 Oct.

Abstract

Supernumerary chromosomes are widespread in the plant kingdom but little is known of their molecular nature or mechanism of origin. We report here the initial cloning of sequences from the maize B chromosome. Our analysis suggests that many sequences are highly repetitive and shared with the normal A chromosomes. However, all clones selected for B-specificity contain at least one copy of a particular repeat. Cytological mapping using B chromosome derivatives and in situ hybridization show that the B specific repeats are derived from the centric region of the chromosome. Sequence analysis of this repeat shows homology to motifs mapped to various plant and animal centromeres and to the maize neocentromere. A precise localization of these sequences among breakpoints within the B centromere and an homology to a facultative centromere, suggest a role for this sequence in centromere function.

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References

    1. Hum Mol Genet. 1992 Dec;1(9):741-7 - PubMed
    1. J Cell Biol. 1991 Jun;113(5):1091-110 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8253-7 - PubMed
    1. Trends Genet. 1990 May;6(5):150-4 - PubMed
    1. Genetics. 1987 Sep;117(1):109-16 - PubMed

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