Heterotypic and homotypic associations between ezrin and moesin, two putative membrane-cytoskeletal linking proteins - PubMed (original) (raw)

Heterotypic and homotypic associations between ezrin and moesin, two putative membrane-cytoskeletal linking proteins

R Gary et al. Proc Natl Acad Sci U S A. 1993.

Abstract

Ezrin and moesin are components of actin-rich cell surface structures that are thought to function as membrane-cytoskeletal linking proteins. Here we show that a stable complex of ezrin and moesin can be isolated from cultured cells by immunoprecipitation with specific antibodies. The capacity of these two proteins to interact directly was confirmed with a blot-overlay procedure in which biotin-tagged proteins in solution were incubated with immobilized binding partners. In addition to the heterotypic association of ezrin and moesin, homotypic binding of ezrin to ezrin and of moesin to moesin was also demonstrated in vitro. These results suggest mechanisms by which ezrin and moesin might participate in dynamic aspects of cortical cytoskeletal structure.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Anal Biochem. 1980 Jul 1;105(2):361-3 - PubMed
    1. J Cell Sci. 1993 Aug;105 ( Pt 4):1025-43 - PubMed
    1. Nature. 1984 Feb 16-22;307(5952):655-8 - PubMed
    1. J Cell Biol. 1986 Feb;102(2):660-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9512-6 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources