A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase - PubMed (original) (raw)
. 1993 Nov 26;262(5138):1407-13.
doi: 10.1126/science.8248780.
Affiliations
- PMID: 8248780
- DOI: 10.1126/science.8248780
A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase
W Ross et al. Science. 1993.
Abstract
A DNA sequence rich in (A+T), located upstream of the -10, -35 region of the Escherichia coli ribosomal RNA promoter rrnB P1 and called the UP element, stimulates transcription by a factor of 30 in vivo, as well as in vitro in the absence of protein factors other than RNA polymerase (RNAP). When fused to other promoters, such as lacUV5, the UP element also stimulates transcription, indicating that it is a separate promoter module. Mutations in the carboxyl-terminal region of the alpha subunit of RNAP prevent stimulation of these promoters by the UP element although the mutant enzymes are effective in transcribing the "core" promoters (those lacking the UP element). Protection of UP element DNA by the mutant RNAPs is severely reduced in footprinting experiments, suggesting that the selective decrease in transcription might result from defective interactions between alpha and the UP element. Purified alpha binds specifically to the UP element, confirming that alpha acts directly in promoter recognition. Transcription of three other promoters was also reduced by the COOH-terminal alpha mutations. These results suggest that UP elements comprise a third promoter recognition region (in addition to the -10, -35 recognition hexamers, which interact with the sigma subunit) and may account for the presence of (A+T)-rich DNA upstream of many prokaryotic promoters. Since the same alpha mutations also block activation by some transcription factors, mechanisms of promoter stimulation by upstream DNA elements and positive control by certain transcription factors may be related.
Similar articles
- Both fis-dependent and factor-independent upstream activation of the rrnB P1 promoter are face of the helix dependent.
Newlands JT, Josaitis CA, Ross W, Gourse RL. Newlands JT, et al. Nucleic Acids Res. 1992 Feb 25;20(4):719-26. doi: 10.1093/nar/20.4.719. Nucleic Acids Res. 1992. PMID: 1542568 Free PMC article. - Molecular anatomy of a transcription activation patch: FIS-RNA polymerase interactions at the Escherichia coli rrnB P1 promoter.
Bokal AJ, Ross W, Gaal T, Johnson RC, Gourse RL. Bokal AJ, et al. EMBO J. 1997 Jan 2;16(1):154-62. doi: 10.1093/emboj/16.1.154. EMBO J. 1997. PMID: 9009276 Free PMC article. - Escherichia coli promoters with UP elements of different strengths: modular structure of bacterial promoters.
Ross W, Aiyar SE, Salomon J, Gourse RL. Ross W, et al. J Bacteriol. 1998 Oct;180(20):5375-83. doi: 10.1128/JB.180.20.5375-5383.1998. J Bacteriol. 1998. PMID: 9765569 Free PMC article. - Transcriptional takeover by sigma appropriation: remodelling of the sigma70 subunit of Escherichia coli RNA polymerase by the bacteriophage T4 activator MotA and co-activator AsiA.
Hinton DM, Pande S, Wais N, Johnson XB, Vuthoori M, Makela A, Hook-Barnard I. Hinton DM, et al. Microbiology (Reading). 2005 Jun;151(Pt 6):1729-1740. doi: 10.1099/mic.0.27972-0. Microbiology (Reading). 2005. PMID: 15941982 Review. - Strength and regulation without transcription factors: lessons from bacterial rRNA promoters.
Gourse RL, Gaal T, Aiyar SE, Barker MM, Estrem ST, Hirvonen CA, Ross W. Gourse RL, et al. Cold Spring Harb Symp Quant Biol. 1998;63:131-9. doi: 10.1101/sqb.1998.63.131. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384277 Review. No abstract available.
Cited by
- Diverse anti-defence systems are encoded in the leading region of plasmids.
Samuel B, Mittelman K, Croitoru SY, Ben Haim M, Burstein D. Samuel B, et al. Nature. 2024 Oct 9. doi: 10.1038/s41586-024-07994-w. Online ahead of print. Nature. 2024. PMID: 39385022 - Clustering of rRNA operons in E. coli is disrupted by σH.
Ho K, Harshey RM. Ho K, et al. bioRxiv [Preprint]. 2024 Sep 21:2024.09.20.614170. doi: 10.1101/2024.09.20.614170. bioRxiv. 2024. PMID: 39345417 Free PMC article. Preprint. - AraC Functional Suppressors of Mutations in the C-Terminal Domain of the RpoA Subunit of the Escherichia coli RNA Polymerase.
Belin D, Costafrolaz J, Silva F. Belin D, et al. Microorganisms. 2024 Sep 23;12(9):1928. doi: 10.3390/microorganisms12091928. Microorganisms. 2024. PMID: 39338602 Free PMC article. - Identification of novel broad host-range promoter sequences functional in diverse Pseudomonadota by a promoter-trap approach.
Roldán DM, Amarelle V. Roldán DM, et al. Braz J Microbiol. 2024 Sep 11. doi: 10.1007/s42770-024-01512-w. Online ahead of print. Braz J Microbiol. 2024. PMID: 39259478 - Identifying widespread and recurrent variants of genetic parts to improve annotation of engineered DNA sequences.
McGuffie MJ, Barrick JE. McGuffie MJ, et al. PLoS One. 2024 May 28;19(5):e0304164. doi: 10.1371/journal.pone.0304164. eCollection 2024. PLoS One. 2024. PMID: 38805426 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases