Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins - PubMed (original) (raw)

. 1993 Dec;7(12A):2378-91.

doi: 10.1101/gad.7.12a.2378.

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• PMID: 8253384
• DOI: 10.1101/gad.7.12a.2378

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Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins

G J Hannon et al. Genes Dev. 1993 Dec.

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Abstract

A two-hybrid protein interaction screen was used to isolate cDNAs encoding human proteins that can interact with human CDK2 in yeast. A new member of the retinoblastoma susceptibility gene family, Rbr-2 (Rb-related), was obtained. The sequence of the Rbr-2 protein shares approximately 50% identify with p107 and homology to Rb within the pocket domain. Several lines of evidence indicate that Rbr-2 is the adenovirus E1A-associated p130. Like Rb and p107, p130Rbr-2 can bind to viral oncoproteins, SV40 large T antigen, and adenovirus E1A through its pocket domain. Although p130Rbr-2 does not bind to CDK2 in vitro, it can interact with cyclins, with a clear preference for D-type cyclins. Because both CDK2 and p130Rbr-2 show affinity for cyclins, we suggest that p130Rbr-2 and CDK2 interacted through a yeast-derived cyclin bridge in the two-hybrid screen. The gene encoding p130Rbr-2 mapped to 16q13, a region of frequent genomic alteration in human tumors.

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