Regulation of human ornithine decarboxylase expression by the c-Myc.Max protein complex - PubMed (original) (raw)
. 1993 Dec 25;268(36):27277-85.
Affiliations
- PMID: 8262968
Free article
Regulation of human ornithine decarboxylase expression by the c-Myc.Max protein complex
A Peña et al. J Biol Chem. 1993.
Free article
Abstract
The presence of a CACGTG element within a region of the human ornithine decarboxylase (ODC) promoter located at -491 to -474 base pairs 5' to the start site of transcription suggested that the c-Myc.Max protein complex may play a role in the regulation of ODC expression during growth. Electrophoretic mobility shift assays and methylation interference analysis showed that the nuclei of WI-38 cells expressing ODC contained proteins that bound to this region of the ODC gene in a manner that correlated with growth-associated ODC expression. Also, use of antibodies against c-Myc and Max and purified recombinant c-Myc and Max protein in the electrophoretic mobility shift assay confirmed that these proteins can specifically bind this portion of the human ODC promoter. Transient transfection studies showed that increase in the level of c-Myc and/or Max led to a significant enhancement of expression of a human ODC promoter-CAT reporter construct. Moreover, treatment of actively growing WI-38 cells with an antisense oligomer to c-Myc reduced the amount of endogenous protein complex formed and the amount of endogenous ODC mRNA expressed. These studies show that the c-Myc.Max protein complex plays a role in the transcriptional regulation of human ODC in vivo.
Similar articles
- Regulation of human ornithine decarboxylase expression following prolonged quiescence: role for the c-Myc/Max protein complex.
Peña A, Wu S, Hickok NJ, Soprano DR, Soprano KJ. Peña A, et al. J Cell Physiol. 1995 Feb;162(2):234-45. doi: 10.1002/jcp.1041620209. J Cell Physiol. 1995. PMID: 7822433 - Overexpression of Mxi1 inhibits the induction of the human ornithine decarboxylase gene by the Myc/Max protein complex.
Wu S, Peña A, Korcz A, Soprano DR, Soprano KJ. Wu S, et al. Oncogene. 1996 Feb 1;12(3):621-9. Oncogene. 1996. PMID: 8637719 - Transcriptional regulation of the ornithine decarboxylase gene by c-Myc/Max/Mad network and retinoblastoma protein interacting with c-Myc.
Auvinen M, Järvinen K, Hotti A, Okkeri J, Laitinen J, Jänne OA, Coffino P, Bergman M, Andersson LC, Alitalo K, Hölttä E. Auvinen M, et al. Int J Biochem Cell Biol. 2003 Apr;35(4):496-521. doi: 10.1016/s1357-2725(02)00305-9. Int J Biochem Cell Biol. 2003. PMID: 12565711 - c-Myc/Max heterodimers bind cooperatively to the E-box sequences located in the first intron of the rat ornithine decarboxylase (ODC) gene.
Walhout AJ, Gubbels JM, Bernards R, van der Vliet PC, Timmers HT. Walhout AJ, et al. Nucleic Acids Res. 1997 Apr 15;25(8):1493-501. doi: 10.1093/nar/25.8.1493. Nucleic Acids Res. 1997. PMID: 9162900 Free PMC article.
Cited by
- Polyamine biosynthesis and eIF5A hypusination are modulated by the DNA tumor virus KSHV and promote KSHV viral infection.
Fiches GN, Wu Z, Zhou D, Biswas A, Li TW, Kong W, Jean M, Santoso NG, Zhu J. Fiches GN, et al. PLoS Pathog. 2022 Apr 29;18(4):e1010503. doi: 10.1371/journal.ppat.1010503. eCollection 2022 Apr. PLoS Pathog. 2022. PMID: 35486659 Free PMC article. - Polyamine Metabolism and Gene Methylation in Conjunction with One-Carbon Metabolism.
Soda K. Soda K. Int J Mol Sci. 2018 Oct 10;19(10):3106. doi: 10.3390/ijms19103106. Int J Mol Sci. 2018. PMID: 30309036 Free PMC article. Review. - Postimplantation Mga expression and embryonic lethality of two gene-trap alleles.
Burn SF, Washkowitz AJ, Gavrilov S, Papaioannou VE. Burn SF, et al. Gene Expr Patterns. 2018 Jan;27:31-35. doi: 10.1016/j.gep.2017.10.006. Epub 2017 Oct 21. Gene Expr Patterns. 2018. PMID: 29066359 Free PMC article. - DFMO: targeted risk reduction therapy for colorectal neoplasia.
Laukaitis CM, Gerner EW. Laukaitis CM, et al. Best Pract Res Clin Gastroenterol. 2011 Aug;25(4-5):495-506. doi: 10.1016/j.bpg.2011.09.007. Best Pract Res Clin Gastroenterol. 2011. PMID: 22122766 Free PMC article. Review. - Growth inhibition of the androgen responsive DDT(1)MF-2 cell line by glucocorticoids: the role of ornithine decarboxylase.
Shubhada S, Soli P, Lamb DJ. Shubhada S, et al. Endocrine. 1995 Jul;3(7):493-8. doi: 10.1007/BF02738823. Endocrine. 1995. PMID: 21153204
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous