Genetic and molecular characterization of embryonic mutants identified following seed transformation in Arabidopsis - PubMed (original) (raw)

. 1993 Dec;241(5-6):504-14.

doi: 10.1007/BF00279892.

Affiliations

• PMID: 8264525
• DOI: 10.1007/BF00279892

Genetic and molecular characterization of embryonic mutants identified following seed transformation in Arabidopsis

L A Castle et al. Mol Gen Genet. 1993 Dec.

Abstract

Over 5000 transgenic families of Arabidopsis thaliana produced following seed transformation with Agrobacterium tumefaciens were screened for embryonic lethals, defectives, and pattern mutants. One hundred and seventy-eight mutants with a wide range of developmental abnormalities were identified. Forty-one mutants appear from genetic studies to be tagged (36% of the 115 mutants examined in detail). Mapping with visible markers demonstrated that mutant genes were randomly distributed throughout the genome. Seven mutant families appeared to contain chromosomal translocations because the mutant genes exhibited linkage to visible markers on two different chromosomes. Chromosomal rearrangements may therefore be widespread following seed transformation. DNA gel blot hybridizations with 34 tagged mutants and three T-DNA probes revealed a wide range of insertion patterns. Models of T-DNA structure at each mutant locus were constructed to facilitate gene isolation. The value of such models was demonstrated by using plasmid rescue to clone flanking plant DNA from four tagged mutants. Further analysis of genes isolated from these insertional mutants should help to elucidate the relationship between gene function and plant embryogenesis.

PubMed Disclaimer

Similar articles

• Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana.
  McElver J, Tzafrir I, Aux G, Rogers R, Ashby C, Smith K, Thomas C, Schetter A, Zhou Q, Cushman MA, Tossberg J, Nickle T, Levin JZ, Law M, Meinke D, Patton D. McElver J, et al. Genetics. 2001 Dec;159(4):1751-63. doi: 10.1093/genetics/159.4.1751. Genetics. 2001. PMID: 11779812 Free PMC article.
• Embryonic Lethals and T-DNA Insertional Mutagenesis in Arabidopsis.
  Errampalli D, Patton D, Castle L, Mickelson L, Hansen K, Schnall J, Feldmann K, Meinke D. Errampalli D, et al. Plant Cell. 1991 Feb;3(2):149-157. doi: 10.1105/tpc.3.2.149. Plant Cell. 1991. PMID: 12324593 Free PMC article.
• Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere.
  Schouten HJ, Vande Geest H, Papadimitriou S, Bemer M, Schaart JG, Smulders MJ, Perez GS, Schijlen E. Schouten HJ, et al. Plant Cell Rep. 2017 Mar;36(3):493-504. doi: 10.1007/s00299-017-2098-z. Epub 2017 Feb 2. Plant Cell Rep. 2017. PMID: 28155116 Free PMC article.
• Arabidopsis thaliana floral dip transformation method.
  Bent A. Bent A. Methods Mol Biol. 2006;343:87-103. doi: 10.1385/1-59745-130-4:87. Methods Mol Biol. 2006. PMID: 16988336 Review.
• A word of caution: T-DNA-associated mutagenesis in plant reproduction research.
  Raabe K, Sun L, Schindfessel C, Honys D, Geelen D. Raabe K, et al. J Exp Bot. 2024 Jun 7;75(11):3248-3258. doi: 10.1093/jxb/erae114. J Exp Bot. 2024. PMID: 38477707 Review.

Cited by

• Genomic consequences associated with Agrobacterium-mediated transformation of plants.
  Thomson G, Dickinson L, Jacob Y. Thomson G, et al. Plant J. 2024 Jan;117(2):342-363. doi: 10.1111/tpj.16496. Epub 2023 Oct 13. Plant J. 2024. PMID: 37831618 Review.
• MicroRNA162 regulates stomatal conductance in response to low night temperature stress via abscisic acid signaling pathway in tomato.
  Li Y, Liu Y, Gao Z, Wang F, Xu T, Qi M, Liu Y, Li T. Li Y, et al. Front Plant Sci. 2023 Mar 2;14:1045112. doi: 10.3389/fpls.2023.1045112. eCollection 2023. Front Plant Sci. 2023. PMID: 36938045 Free PMC article.
• Characterization of T-Circles and Their Formation Reveal Similarities to Agrobacterium T-DNA Integration Patterns.
  Singer K, Lee LY, Yuan J, Gelvin SB. Singer K, et al. Front Plant Sci. 2022 May 6;13:849930. doi: 10.3389/fpls.2022.849930. eCollection 2022. Front Plant Sci. 2022. PMID: 35599900 Free PMC article.
• Plant DNA Repair and Agrobacterium T-DNA Integration.
  Gelvin SB. Gelvin SB. Int J Mol Sci. 2021 Aug 6;22(16):8458. doi: 10.3390/ijms22168458. Int J Mol Sci. 2021. PMID: 34445162 Free PMC article. Review.
• Large scale genomic rearrangements in selected Arabidopsis thaliana T-DNA lines are caused by T-DNA insertion mutagenesis.
  Pucker B, Kleinbölting N, Weisshaar B. Pucker B, et al. BMC Genomics. 2021 Aug 6;22(1):599. doi: 10.1186/s12864-021-07877-8. BMC Genomics. 2021. PMID: 34362298 Free PMC article.

References

1. 1. Mol Gen Genet. 1991 Jul;227(3):337-47 - PubMed
2. 1. Cell. 1992 Nov 27;71(5):791-801 - PubMed
3. 1. Nature. 1990 Jul 5;346(6279):35-9 - PubMed
4. 1. Plant Mol Biol. 1990 Dec;15(6):851-64 - PubMed
5. 1. Theor Appl Genet. 1985 Mar;69(5-6):543-52 - PubMed

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Springer

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • The Arabidopsis Information Resource