Induction of type I interferon genes and interferon-inducible genes in embryonal stem cells devoid of interferon regulatory factor 1 - PubMed (original) (raw)

Induction of type I interferon genes and interferon-inducible genes in embryonal stem cells devoid of interferon regulatory factor 1

H Ruffner et al. Proc Natl Acad Sci U S A. 1993.

Abstract

Overexpression of interferon regulatory factor 1 (IRF-1) can induce expression of the interferon (IFN) beta gene, at least in certain cells. A role of IRF-1 in the activation of IFN-alpha genes has also been claimed. We have generated embryonal stem cells in which both IRF-1 alleles were disrupted. In undifferentiated embryonal stem cells, virus-induced levels of IFN-alpha RNA were similar for wild-type and IRF-1%, and there was little induction of IFN-beta RNA in either cell type. In 8-day differentiated cells, the levels of virus-induced IFN-beta RNA, but not of IFN-alpha RNA, were about 10-fold higher than in undifferentiated cells and only slightly higher in wild-type than in IRF-1% cells. Thus, although IRF-1 at high levels may elicit or augment induction of IFN genes under certain circumstances, it is not essential for IFN gene induction by virus. Lack of IRF-1 had no effect on the IFN-induced expression levels of the IFN-inducible genes tested; however, there was little or no constitutive expression of (2'-5')oligoadenylate synthetase in IRF-1% embryonal stem cells, in contrast to wild-type cells.

PubMed Disclaimer

Cited by

Delayed embryonic lethality in mice lacking protein phosphatase 2A catalytic subunit Calpha.
Götz J, Probst A, Ehler E, Hemmings B, Kues W. Götz J, et al. Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12370-5. doi: 10.1073/pnas.95.21.12370. Proc Natl Acad Sci U S A. 1998. PMID: 9770493 Free PMC article.
Mice with combined gene knock-outs reveal essential and partially redundant functions of amyloid precursor protein family members.
Heber S, Herms J, Gajic V, Hainfellner J, Aguzzi A, Rülicke T, von Kretzschmar H, von Koch C, Sisodia S, Tremml P, Lipp HP, Wolfer DP, Müller U. Heber S, et al. J Neurosci. 2000 Nov 1;20(21):7951-63. doi: 10.1523/JNEUROSCI.20-21-07951.2000. J Neurosci. 2000. PMID: 11050115 Free PMC article.
An Sp1 binding site and the minimal promoter contribute to overexpression of the cytokeratin 18 gene in tumorigenic clones relative to that in nontumorigenic clones of a human carcinoma cell line.
Gunther M, Frebourg T, Laithier M, Fossar N, Bouziane-Ouartini M, Lavialle C, Brison O. Gunther M, et al. Mol Cell Biol. 1995 May;15(5):2490-9. doi: 10.1128/MCB.15.5.2490. Mol Cell Biol. 1995. PMID: 7537848 Free PMC article.
A factor IX-deficient mouse model for hemophilia B gene therapy.
Wang L, Zoppè M, Hackeng TM, Griffin JH, Lee KF, Verma IM. Wang L, et al. Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11563-6. doi: 10.1073/pnas.94.21.11563. Proc Natl Acad Sci U S A. 1997. PMID: 9326649 Free PMC article.
Differential viral induction of distinct interferon-alpha genes by positive feedback through interferon regulatory factor-7.
Marié I, Durbin JE, Levy DE. Marié I, et al. EMBO J. 1998 Nov 16;17(22):6660-9. doi: 10.1093/emboj/17.22.6660. EMBO J. 1998. PMID: 9822609 Free PMC article.

References

1. EMBO J. 1993 Feb;12(2):451-9 - PubMed
1. J Interferon Res. 1992 Dec;12(6):445-8 - PubMed
1. Cell. 1985 Jun;41(2):509-20 - PubMed
1. Nucleic Acids Res. 1985 Mar 11;13(5):1431-42 - PubMed
1. J Biol Chem. 1986 Sep 5;261(25):11849-58 - PubMed

Induction of type I interferon genes and interferon-inducible genes in embryonal stem cells devoid of interferon regulatory factor 1 - PubMed (original) (raw)