Induction of erythroid-specific gene expression in lymphoid cells - PubMed (original) (raw)

Induction of erythroid-specific gene expression in lymphoid cells

T Chiba et al. Proc Natl Acad Sci U S A. 1993.

Abstract

Erythropoietin (Epo) is a cytokine which specifically regulates differentiation and proliferation of erythroid progenitor cells. We report here that Epo receptor expressed in interleukin 3-dependent lymphoid Ba/F3 cells transmits both differentiation and growth signals. Epo stimulation of these cells leads to activation of transcription and/or translation of the erythroid-specific transcription factors GATA-1 and SCL, followed by the accumulation of both alpha- and beta-globin chains. These results suggest that expression and activation of the Epo receptor regulates erythroid-specific gene expression and might play a role in determining a cell lineage in vivo and that GATA-1 and SCL may exert their effects after Epo binds to its receptor. It was further found that chimeric receptors composed of extracellular domains of Epo receptor and cytoplasmic domains of interleukin 2 or interleukin 3 receptors could also induce erythroid-specific gene expression in Ba/F3 cells. Taking these data together with previous observations, we conclude that interaction of the extracellular domains of the Epo receptor with other membrane components is essential for transmission of both the erythroid differentiation and the growth signals.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 - PubMed
    1. Nature. 1993 Apr 15;362(6421):646-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4126-30 - PubMed
    1. Anal Biochem. 1987 Apr;162(1):156-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Dec;86(24):10128-32 - PubMed

MeSH terms

Substances

LinkOut - more resources