Involvement of U6 snRNA in 5' splice site selection - PubMed (original) (raw)
. 1993 Dec 24;262(5142):2035-9.
doi: 10.1126/science.8266100.
Affiliations
- PMID: 8266100
- DOI: 10.1126/science.8266100
Involvement of U6 snRNA in 5' splice site selection
S Kandels-Lewis et al. Science. 1993.
Erratum in
- Science 1994 Feb 11;263(5148):739
Abstract
Two models describing the interaction between U6 small nuclear RNA (snRNA) and the 5' splice site of introns have been proposed on the basis of cross-linking experiments. Here it is shown that a conserved sequence present in U6 snRNA forms base pairs with conserved nucleotides at the 5' splice junction and that this interaction is involved in 5' splice site choice. These results demonstrate a specific function for U6 snRNA in splicing and suggest that U6 snRNA has a proofreading role during splice site selection. A model is presented in which this new interaction, in concert with previously described interactions between U6 snRNA, U2 snRNA, and the pre-messenger RNA, would position the branch point near the 5' splice site for the catalysis of the first splicing step.
Comment in
- Guides to the heart of the spliceosome.
Wise JA. Wise JA. Science. 1993 Dec 24;262(5142):1978-9. doi: 10.1126/science.8266091. Science. 1993. PMID: 8266091 Review. No abstract available.
Similar articles
- Genetic interaction between U6 snRNA and the first intron nucleotide in Saccharomyces cerevisiae.
Luukkonen BG, Séraphin B. Luukkonen BG, et al. RNA. 1998 Feb;4(2):167-80. RNA. 1998. PMID: 9570316 Free PMC article. - Mutations in U6 snRNA that alter splice site specificity: implications for the active site.
Lesser CF, Guthrie C. Lesser CF, et al. Science. 1993 Dec 24;262(5142):1982-8. doi: 10.1126/science.8266093. Science. 1993. PMID: 8266093 - Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing.
Xu D, Field DJ, Tang SJ, Moris A, Bobechko BP, Friesen JD. Xu D, et al. Mol Cell Biol. 1998 Apr;18(4):2055-66. doi: 10.1128/MCB.18.4.2055. Mol Cell Biol. 1998. PMID: 9528778 Free PMC article. - The life of U6 small nuclear RNA, from cradle to grave.
Didychuk AL, Butcher SE, Brow DA. Didychuk AL, et al. RNA. 2018 Apr;24(4):437-460. doi: 10.1261/rna.065136.117. Epub 2018 Jan 24. RNA. 2018. PMID: 29367453 Free PMC article. Review. - Who's on first? The U1 snRNP-5' splice site interaction and splicing.
Rosbash M, Séraphin B. Rosbash M, et al. Trends Biochem Sci. 1991 May;16(5):187-90. doi: 10.1016/0968-0004(91)90073-5. Trends Biochem Sci. 1991. PMID: 1882420 Review.
Cited by
- Functional Analysis of the Zinc Finger Modules of the S. cerevisiae Splicing Factor Luc7.
Carrocci TJ, DeMario S, He K, Zeps NJ, Harkner CT, Chanfreau G, Hoskins AA. Carrocci TJ, et al. bioRxiv [Preprint]. 2024 Feb 4:2024.02.04.578419. doi: 10.1101/2024.02.04.578419. bioRxiv. 2024. PMID: 38352541 Free PMC article. Updated. Preprint. - Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs.
Kramárek M, Souček P, Réblová K, Grodecká LK, Freiberger T. Kramárek M, et al. Nucleic Acids Res. 2024 Jun 10;52(10):5959-5974. doi: 10.1093/nar/gkae147. Nucleic Acids Res. 2024. PMID: 38426935 Free PMC article. - From the ribosome to the spliceosome and back again.
Guthrie C. Guthrie C. J Biol Chem. 2010 Jan 1;285(1):1-12. doi: 10.1074/jbc.X109.080580. Epub 2009 Oct 30. J Biol Chem. 2010. PMID: 19880518 Free PMC article. No abstract available. - Multiple functions for the invariant AGC triad of U6 snRNA.
Hilliker AK, Staley JP. Hilliker AK, et al. RNA. 2004 Jun;10(6):921-8. doi: 10.1261/rna.7310704. RNA. 2004. PMID: 15146076 Free PMC article. - U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing.
Fukumura K, Taniguchi I, Sakamoto H, Ohno M, Inoue K. Fukumura K, et al. Nucleic Acids Res. 2009 Apr;37(6):1907-14. doi: 10.1093/nar/gkp050. Epub 2009 Feb 3. Nucleic Acids Res. 2009. PMID: 19190090 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources