Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope - PubMed (original) (raw)

. 1993 Aug;118(4):1255-66.

doi: 10.1242/dev.118.4.1255.

Affiliations

• PMID: 8269852
• DOI: 10.1242/dev.118.4.1255

Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope

K M Downs et al. Development. 1993 Aug.

Abstract

We describe and illustrate a set of morphological landmarks for classifying mouse embryos by gross morphology from before gastrulation to the beginning of organogenesis. These landmarks are visible at the resolution of the dissecting microscope in embryos that are intact except for reflexion of Reichert's membrane. Adoption of these criteria should facilitate interpretation of both the expression patterns of genes and the consequences of experimental manipulation of embryos during early postimplantation development.

PubMed Disclaimer

Similar articles

• Possible role of the 38 kDa protein, lacking in the gastrula-arrested Xenopus mutant, in gastrulation.
  Tanaka TS, Ikenishi K. Tanaka TS, et al. Dev Growth Differ. 2002 Feb;44(1):23-33. doi: 10.1046/j.1440-169x.2002.00619.x. Dev Growth Differ. 2002. PMID: 11869289
• H beta 58, an insertional mutation affecting early postimplantation development of the mouse embryo.
  Radice G, Lee JJ, Costantini F. Radice G, et al. Development. 1991 Mar;111(3):801-11. doi: 10.1242/dev.111.3.801. Development. 1991. PMID: 1879343
• Intrauterine Pressures Adjusted by Reichert's Membrane Are Crucial for Early Mouse Morphogenesis.
  Ueda Y, Kimura-Yoshida C, Mochida K, Tsume M, Kameo Y, Adachi T, Lefebvre O, Hiramatsu R, Matsuo I. Ueda Y, et al. Cell Rep. 2020 May 19;31(7):107637. doi: 10.1016/j.celrep.2020.107637. Cell Rep. 2020. PMID: 32433954
• Gastrulation in the mouse embryo: ultrastructural and molecular aspects of germ layer morphogenesis.
  Tam PP, Williams EA, Chan WY. Tam PP, et al. Microsc Res Tech. 1993 Nov 1;26(4):301-28. doi: 10.1002/jemt.1070260405. Microsc Res Tech. 1993. PMID: 8305722 Review.
• The natural variability of morphogenesis: a tool for exploring the mechanics of gastrulation movements in amphibian embryos.
  Scobeyeva VA. Scobeyeva VA. Int J Dev Biol. 2006;50(2-3):315-22. doi: 10.1387/ijdb.052062vs. Int J Dev Biol. 2006. PMID: 16479499 Review.

Cited by

• New findings on the orientation of the mouse anterior-posterior (A-P) axis before and during the initiation of gastrulation using a more refined embryo staging.
  Hadjikypri X, Theofanous C, Christodoulidi A, Georgiades P. Hadjikypri X, et al. Biochem Biophys Rep. 2024 Aug 29;40:101817. doi: 10.1016/j.bbrep.2024.101817. eCollection 2024 Dec. Biochem Biophys Rep. 2024. PMID: 39286290 Free PMC article.
• Gastrulation-stage gene expression in Nipbl+/- mouse embryos foreshadows the development of syndromic birth defects.
  Chea S, Kreger J, Lopez-Burks ME, MacLean AL, Lander AD, Calof AL. Chea S, et al. Sci Adv. 2024 Mar 22;10(12):eadl4239. doi: 10.1126/sciadv.adl4239. Epub 2024 Mar 20. Sci Adv. 2024. PMID: 38507484 Free PMC article.
• Tracking early mammalian organogenesis - prediction and validation of differentiation trajectories at whole organism scale.
  Imaz-Rosshandler I, Rode C, Guibentif C, Harland LTG, Ton MN, Dhapola P, Keitley D, Argelaguet R, Calero-Nieto FJ, Nichols J, Marioni JC, de Bruijn MFTR, Göttgens B. Imaz-Rosshandler I, et al. Development. 2024 Feb 1;151(3):dev201867. doi: 10.1242/dev.201867. Epub 2024 Jan 31. Development. 2024. PMID: 37982461 Free PMC article.
• Selective Inhibition of mTORC1 Signaling Supports the Development and Maintenance of Pluripotency.
  Kim JK, Villa-Diaz LG, Saunders TL, Saul RP, Timilsina S, Liu F, Mishina Y, Krebsbach PH. Kim JK, et al. Stem Cells. 2024 Jan 13;42(1):13-28. doi: 10.1093/stmcls/sxad079. Stem Cells. 2024. PMID: 37931173
• Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation.
  Wang R, Yang X, Chen J, Zhang L, Griffiths JA, Cui G, Chen Y, Qian Y, Peng G, Li J, Wang L, Marioni JC, Tam PPL, Jing N. Wang R, et al. Nat Commun. 2023 Sep 14;14(1):5675. doi: 10.1038/s41467-023-41482-5. Nat Commun. 2023. PMID: 37709743 Free PMC article.

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)