Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region - PubMed (original) (raw)
Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region
J P Montmayeur et al. Science. 1994.
Abstract
Heterotrimeric guanosine triphosphate (GTP)-binding proteins (G proteins) may participate in membrane traffic events. A complementary DNA (cDNA) was isolated from a mouse pituitary cDNA library that corresponded to an alternatively spliced form of the gene encoding the G protein alpha subunit G alpha i2. The cDNA was identical to that encoding G alpha i2 except that the region encoding for the carboxyl-terminal 24 amino acids was replaced by a longer region encoding 35 amino acids that have no sequence similarity with G alpha i2 or other members of the G protein family. This alternative spliced product and the corresponding protein (sGi2) were present in several tissues. Specific antibodies revealed that sGi2 was localized in the Golgi apparatus, suggesting a role in membrane transport. Thus, alternative splicing may generate from a single gene two G protein alpha subunits with differential cellular localization and function.
Similar articles
- A region containing a proline-rich motif targets sG(i2) to the golgi apparatus.
Picetti R, Borrelli E. Picetti R, et al. Exp Cell Res. 2000 Mar 15;255(2):258-69. doi: 10.1006/excr.1999.4783. Exp Cell Res. 2000. PMID: 10694441 - Selective interactions of mu-opioid receptors with pertussis toxin-sensitive G proteins: involvement of the third intracellular loop and the c-terminal tail in coupling.
Georgoussi Z, Merkouris M, Mullaney I, Megaritis G, Carr C, Zioudrou C, Milligan G. Georgoussi Z, et al. Biochim Biophys Acta. 1997 Dec 12;1359(3):263-74. doi: 10.1016/s0167-4889(97)00097-9. Biochim Biophys Acta. 1997. PMID: 9434132 - Regulation of the golgi structure by the alpha subunits of heterotrimeric G proteins.
Yamaguchi T, Nagahama M, Itoh H, Hatsuzawa K, Tani K, Tagaya M. Yamaguchi T, et al. FEBS Lett. 2000 Mar 17;470(1):25-8. doi: 10.1016/s0014-5793(00)01284-9. FEBS Lett. 2000. PMID: 10722839 - Genetic analysis of mammalian G-protein signalling.
Offermanns S, Simon MI. Offermanns S, et al. Oncogene. 1998 Sep 17;17(11 Reviews):1375-81. doi: 10.1038/sj.onc.1202173. Oncogene. 1998. PMID: 9779985 Review. - Distribution and role of heterotrimeric G proteins in the secretory pathway of polarized epithelial cells.
Stow JL, de Almeida JB. Stow JL, et al. J Cell Sci Suppl. 1993;17:33-9. doi: 10.1242/jcs.1993.supplement_17.6. J Cell Sci Suppl. 1993. PMID: 8144703 Review.
Cited by
- Gαi protein subunit: A step toward understanding its non-canonical mechanisms.
Villaseca S, Romero G, Ruiz MJ, Pérez C, Leal JI, Tovar LM, Torrejón M. Villaseca S, et al. Front Cell Dev Biol. 2022 Aug 24;10:941870. doi: 10.3389/fcell.2022.941870. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36092739 Free PMC article. Review. - Mitochondrial H2O2 as an enable signal for triggering autophosphorylation of insulin receptor in neurons.
Persiyantseva NA, Storozhevykh TP, Senilova YE, Gorbacheva LR, Pinelis VG, Pomytkin IA. Persiyantseva NA, et al. J Mol Signal. 2013 Oct 5;8(1):11. doi: 10.1186/1750-2187-8-11. J Mol Signal. 2013. PMID: 24094269 Free PMC article. - Identification and experimental validation of G protein alpha inhibiting activity polypeptide 2 (GNAI2) as a microRNA-138 target in tongue squamous cell carcinoma.
Jiang L, Dai Y, Liu X, Wang C, Wang A, Chen Z, Heidbreder CE, Kolokythas A, Zhou X. Jiang L, et al. Hum Genet. 2011 Feb;129(2):189-97. doi: 10.1007/s00439-010-0915-3. Epub 2010 Nov 16. Hum Genet. 2011. PMID: 21079996 Free PMC article. - The physiology of membrane transport and endomembrane-based signalling.
Sallese M, Pulvirenti T, Luini A. Sallese M, et al. EMBO J. 2006 Jun 21;25(12):2663-73. doi: 10.1038/sj.emboj.7601172. Epub 2006 Jun 8. EMBO J. 2006. PMID: 16763561 Free PMC article. Review. - Characterization of subcellular localization and stability of a splice variant of G alpha i2.
Wedegaertner PB. Wedegaertner PB. BMC Cell Biol. 2002 May 31;3:12. doi: 10.1186/1471-2121-3-12. BMC Cell Biol. 2002. PMID: 12057015 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources