zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly - PubMed (original) (raw)

zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly

O Kuge et al. J Cell Biol. 1993 Dec.

Abstract

cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon-COP), zeta-COP exists in both coatomer bound and free pools.

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