Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification - PubMed (original) (raw)

Comparative Study

. 1993 Nov;2(11):1907-14.

doi: 10.1093/hmg/2.11.1907.

Affiliations

• PMID: 8281155
• DOI: 10.1093/hmg/2.11.1907

Comparative Study

Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification

M R Speicher et al. Hum Mol Genet. 1993 Nov.

Abstract

We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications in archived solid tumor samples.

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