Translation initiation factor IF1 is essential for cell viability in Escherichia coli - PubMed (original) (raw)

Translation initiation factor IF1 is essential for cell viability in Escherichia coli

H S Cummings et al. J Bacteriol. 1994 Jan.

Abstract

Translation initiation factor IF1 is a highly conserved element of the prokaryotic translational apparatus. It has been demonstrated earlier that the factor stimulates in vitro the initiation phase of protein synthesis. However, no mutation in its gene, infA, has been identified, and a role for IF1 in translation has not been demonstrated in vivo. To elucidate the function of IF1 and determine if the protein is essential for cell growth, the chromosomal copy of infA was disrupted. Cell viability is maintained only when infA is expressed in trans from a plasmid, thereby demonstrating that IF1 is essential for cell growth in Escherichia coli. Cells depleted of IF1 exhibit few polysomes, suggesting that IF1 functions in the initiation phase of protein synthesis.

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References

    1. Biochemistry. 1986 Jun 17;25(12):3682-90 - PubMed
    1. Nucleic Acids Res. 1986 Jan 24;14(2):1029-44 - PubMed
    1. J Mol Biol. 1976 Jan 5;100(1):35-45 - PubMed
    1. J Bacteriol. 1977 Jan;129(1):378-87 - PubMed
    1. Anal Biochem. 1987 Nov 1;166(2):368-79 - PubMed

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