Crystal structure of the DNA binding domain of the heat shock transcription factor - PubMed (original) (raw)

. 1994 Jan 14;263(5144):224-7.

doi: 10.1126/science.8284672.

Affiliations

• PMID: 8284672
• DOI: 10.1126/science.8284672

Crystal structure of the DNA binding domain of the heat shock transcription factor

C J Harrison et al. Science. 1994.

Abstract

The structure of the DNA binding domain, determined at 1.8 angstrom resolution, contains a three-helix bundle that is capped by a four-stranded antiparallel beta sheet. This structure is a variant of the helix-turn-helix motif, typified by catabolite activator protein. In the heat shock transcription factor, the first helix of the motif (alpha 2) has an alpha-helical bulge and a proline-induced kink. The angle between the two helices of the motif (alpha 2 and alpha 3) is about 20 degrees smaller than the average for canonical helix-turn-helix proteins. Nevertheless, the relative positions of the first and third helices of the bundle (alpha 1 and alpha 3) are conserved. It is proposed here that the first helix of the three-helix bundle be considered a component of the helix-turn-helix motif.

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